参附注射液对缺氧缺血性脑损伤新生大鼠白介素-18和白介素-1β的影响  

Effects of Shenfu Injection on the expression of Interleukin-18 and Interleukin-1β in neonatal rats with Hypoxic-ischemic brain damage

作　　者：徐艳[1] 陈群娥[1] 王军[1] 王伟[1] 张文辉[1] 周长美[1] 朱红梅[1] 张绍美[1] 

机构地区：[1]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《南京医科大学学报（自然科学版）》2009年第4期479-482,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅科技计划重大项目基金资助(H200823)

摘　　要：目的:探讨参附注射液(SFI)对缺氧缺血性脑损伤(HIBD)新生大鼠白细胞介素-18(IL-18)和白细胞介素-1β(IL-1β)的影响。方法:参照Rice法制备新生大鼠HIBD模型,将新生7日龄SD大鼠随机分为假手术组(S)、生理盐水对照组(C)、参附治疗组(SF),每组按术后观察时间点不同进一步分为3、12、24h、6、14天5个亚组,用ELISA方法检测病变侧脑组织中IL-18、IL-1β水平。结果:C组和SF组IL-1β(12、24h、6天)、IL-18(24、6、14天)表达水平均较S组升高,其差别均有统计学意义(P<0.01);IL-1β在HI后12h即达到高峰,随后开始下降,14天已降至正常水平。IL-18在HI后24h开始增加,6天达到高峰,之后开始下降,14天仍高于S组(P<0.01)。SF组IL-1β表达水平在12、24h、6天较C组降低,其差别均有统计学意义(P<0.01);SF组IL-18表达水平在24h、6、14天较C组减少,其差别均有统计学意义(P<0.01)。结论:HIBD后大鼠病变侧大脑皮层IL-18、IL-1β水平明显升高,参附注射液(SFI)下调HIBD新生大鼠IL-18和IL-1β的表达水平,SFI对新生大鼠HIBD有保护作用。Objective：To investigate the effect of Shenfu injection （SFI） on the expression of Interleukin-18 （IL-18） and Inter- leukin-1β（IL-1β） in neonatal rats with hypoxic - ischemic brain damage（HIBD）, and clarify the protection of SFI for HI brain damage at the molecular level. Methods：Seven-day-old Sprague-Dawley rats were randomly assigned to sham operation group（S）, normal sodium control group （C） and Shenfu injection treatment group （SF）, and Models of postnatal 7-day Sprague Dawley （SD） rats with HIBD were established by the Rice method. They were further divided into five subgroups（n = 8 per subgroup） according to the time points 3 h, 12 h,24 h,6d and 14 d. In each subgroup, rats were used to examine the level of IL-18,IL-1β in brain homogenates by ELISA. Results：The level of IL-1β was significantly higher in group C and group SF higher than that in group S at the time point 12 h, 24 h, 6 d （P 〈 0.01 ）; The levels of IL-18 in group C and group SF were significantly higher than that of group S at the time point 24 h, 6 d, 14 d（P 〈 0.01 ） ; The expression of IL-1β in group C peaked at 12 h after HI insult and decreased after this, There was no difference in group S and group C at 14 d （P 〉 0.05）; The expression of IL-18 in group C began to increase at 24 h after HI insult（P 〈 0.01 ）, peaked at 6 d after HI insult and decreased after this. But IL-18 was still higher than group S （P 〈 0.01） at 14 d; the level of IL-1β in brain homogenates after HI insult in group SF were lower than group C at 12 h,24 h,6 d （P 〈 0.01） ; the level of IL-18 in brain homogenates after HI insult in group SF were lower than group C at every observed spot after 12 h （P 〈 0.01 ）. Conclusion：The level of IL-18 and IL-1β in cerebral cortex had increased after HI insult in neonatal rats and SFI can decrease the level of IL-18 and IL-1β in cerebral cortex. SFI has a protedive function in neonatal rats after HI insult.

关 键 词：白细胞介素-18 白细胞介素-1β 缺氧缺血性脑损伤 新生大鼠 参附注射液 

分 类 号：R722.12[医药卫生—儿科]