蒙古黄芪的组织培养及辐射诱变研究  

Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao

作　　者：赵秀娟[1] 张红霞[1] 

机构地区：[1]内蒙古科技大学生物与化学工程学院,内蒙古包头014010

出　　处：《安徽农业科学》2009年第9期3939-3941,共3页Journal of Anhui Agricultural Sciences

基　　金：内蒙古科技大学校内基金项目(KJ200611)

摘　　要：[目的]研究蒙古黄芪的组织培养技术及辐射诱变对愈伤组织诱导的影响。[方法]以蒙古黄芪无菌苗的不同外植体(叶片、子叶、下胚轴)进行愈伤组织诱导,并对子叶和下胚轴进行辐射诱变研究。[结果]3种外植体分别在MS+6-BA 2.0 mg/L+NAA 2.0 mg/L、LS+6-BA 2.0 mg/L+NAA 0.1 mg/L、MS+6-BA 2.0 mg/L+NAA 2.0 mg/L培养基上的愈伤组织诱导率较高;下胚轴和子叶的最佳诱变时间均为15 min;随辐射诱变时间的增加,下胚轴愈伤组织的长势增强,但超过一定的诱变时间愈伤组织长势减弱且诱导率降低。[结论]该研究为蒙古黄芪的组织培养和诱变育种提供科学依据。[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of Astragalus membranaceus Bge.var.mongholicus（Bge.） Hsiao.[Method] With the different parts of Astragalus membranaceus Bge.var.mongholicus（Bge.） Hsiao aseptic seedling as explants（leaves,cotyledons,hypocotyls） induced callus,and cotyledon and hypocotyls taken by the method of radiation mutation were studied.[Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS ＋ 6-BA 2.0 mg/L ＋ NAA 2.0 nag/L, KS ＋ 6-BA 2.0 mg/L ＋ NAA 0.1 mg/L, MS ＋ 6-BA 2.0 mg/L ＋ NAA 2.0 mg/L; the best mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. [Coaclusion]This study will provide the scientific reference in tissue culture and mutation breeding of Astragalus membnnaceus Bge. var. mongholicus （Bge.） Hsiao.

关 键 词：蒙古黄芪 组织培养 辐射诱变 

分 类 号：S603.6[农业科学—园艺学]