绵羊肌肉生长激素受体基因表达的发育性变化研究  

Developmental Changes of Muscular GHR mRNA Expression in Sheep

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《安徽农业科学》2009年第9期3977-3979,共3页Journal of Anhui Agricultural Sciences

基　　金：新疆生产建设兵团博士基金(2003-02)

摘　　要：[目的]探讨绵羊肌肉生长激素受体基因（GHR）在生长发育早期的表达。[方法]用real time PCR法对不同日龄雄性哈萨克羊和新疆细毛羊背最长肌GHRmRNA的表达进行定量分析。[结果]绵羊背最长肌GHRmRNA的表达随着日龄的增加先升后降,然后趋于水平,30日龄时最高。哈萨克羊的表达量在2~90日龄期间都极显著低于新疆细毛羊（P〈0.01）。[结论]日龄和品种对绵羊肌肉GHR基因表达均有较大影响。[Objective] The study aimed to explore the expression of muscular growth hormone receptor gene（GHR） in sheep at the early stage of growth and development.[Method] The expression levels of longissimus dorsal muscular GHR mRNA in male Kazak sheep and Xinjiang fine wool sheep with different ages were quantitatively analyzed by real time PCR.[Result] Sheep GHR mRNA expression level in longissimus dorsal muscle increased firstly followed by decline, and then kept steady until thes end of the experiment, with the expression peak appearing on postnatal day 30. The GHR mRNA expression level of Kazak sheep was extremely lower than that of Xingjiang fine wool sheep from 2 to 90 days old （ P 〈0.01 ）. [ Conclusion] Both age and bread have great effects on the expression of muscular GHR gene in sheep.

关 键 词：绵羊 肌肉 生长激素受体基因 表达 

分 类 号：S826[农业科学—畜牧学]