热应激对小鼠垂体前叶细胞构筑的影响  

Effect of Heat Stress on the Cytoarchitecture of Pituitary Gland Anterior Lobe in Mouse

作　　者：尹锋[1] 陈光英[2] 余丝莉[1] 李中文[1] 刘佳[1] 李继东[1] 贾志君[1] 陈忠[1,2] 

机构地区：[1]海南师范大学生命科学学院,海南海口571185 [2]海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《安徽农业科学》2009年第9期4052-4053,4104,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30660137);海南省教育厅科研基金(Hjkj2009-44)

摘　　要：[目的]通过在急性热应激条件下对小鼠垂体前叶细胞构筑变化的观察与测量,确定热应激对小鼠垂体前叶细胞构筑的影响。[方法]采用恒温恒湿热应激箱(42℃,相对湿度35.4%)建立热应激模型。常规石蜡切片,HE染色,在光镜下对垂体前叶细胞构筑进行观察。[结果]热应激组Ⅰ、Ⅱ的细胞密度、嗜碱性细胞概率、嗜碱性细胞体面积以及热应激组Ⅱ嗜酸性细胞体面积与对照组差异极显著(P<0.001)。热应激组Ⅰ嗜酸性细胞概率、嗜酸性细胞体面积与对照组差异显著(P<0.05)。[结论]热应激可导致垂体前叶细胞快速增殖和正常形态发生改变,甚至引发细胞凋亡。[Objective] The research aimed to determine the impact of heat stress on the cytoarchitecture of anterior pituitary,observe and measure the anterior pituitary cells of mouse under the acute conditions of heat stress.[Method]Different temperature of heat stress was Created in a constant temperature and humidity box（42 ℃,relative humidity of 35.4%）.The Conventional of paraffin slice was Stained by HE and it was observed by light microscope.[Result] The statistical data indicate that the cellular density.the proportion and the acreage of Basophils were very significant different with the control group in HSI and ]]（ P 〈 0.001）, the acreage of acidophil were very sigifificant different with the control group in HS]]（ P 〈 0.001 ）, the proportion and the acreage of acidaphil were significant different with the control group in HSI（ P 〈 0.05）. [ Conclusion]A rapid proliferation of cell was stimulated and the celluar normal morphology was changed after heat stress, even trigger apoptosis.

关 键 词：热应激 垂体 细胞构筑 小鼠 

分 类 号：Q426[生物学—神经生物学]