Isolation and characterization of sexual dimorphism genes expressed in chicken embryonic gonads  

作　　者：Yanping Feng Xiuli Peng Shijun Li Yanzhang Gong 

机构地区：[1]Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan430070, China

出　　处：《Acta Biochimica et Biophysica Sinica》2009年第4期285-294,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （30671494）.

摘　　要：In chicken, the bipotential embryonic gonad differentiates into either a pair of testes or an ovary, but few genes that underlying the gonadal sex differentiation have been identified and the sex-determination gene is still unknown. To identify more genes involved in chicken sex differentiation, we employed suppression subtractive hybridization to isolate differentially expressed genes between sexes from chicken gonads during a period of E3.5-E6. A total of 152 cDNA clones corresponding to 88 genes （41 from F-M library and 47 from M-F library） were screened using dot-blot analysis. These genes are located mainly on the macrochromosomes （1-5） with five in the sex chromosomes （one in W and four in Z）, encoding four dominating molecular categories belonging to enzyme, DNA association, RNA association, and structural protein. Comparing the obtained cDNA sequences with those in chicken EST database, it showed that cDNAs of 32 genes from F-M library and 16 from M-F library have homologs in two reported embryonic gonad cDNA libraries. Quantitative real-time PCR analysis of eight genes involved in epigenetic and transcription regu- lation showed significantly different expression between sexes of CDK2AP1, SMARCEI, SAP18, SUDS3, and PQBP1 appeared at the early stage in gonad development （E4）. Based on the functional comparison of sexual differentially expressed genes, the roles of some putatively important genes including ATP5A1 W, CDK2API, mitochondrial transcripts, etc. have been analyzed. In conclusion, characterization of isolated genes would provide valuable clues to identify potential candidates involved in genetic mechanisms of chicken sex differentiation and gonad development.在鸡肉，胚胎的性腺区分进一双睾丸或一个卵巢的 bipotential，而是位于 gonadal 性区别下面被识别了的很少基因和性决心基因仍然是未知的。为了识别更多的基因，在鸡性区别包含了，我们采用了孤立差别的减少性的杂交表示了的抑制在从鸡性腺的性之间的基因在 E3.5E6 的一个时期期间。相应于 88 基因的 152 cDNA 克隆的一个总数(41 从 F-M 图书馆并且 47 从 M-F 图书馆) 用点污点分析被屏蔽。这些基因在性染色体与五主要位于宏染色体(15 )( 在 W 的并且四在 Z ) ，编码四统治属于酶， DNA 协会， RNA 协会，和结构的蛋白质的分子的范畴。在鸡 EST 数据库把获得的 cDNA 序列与那些作比较，它从 F-M 图书馆显示出 32 基因的那 cDNAs 并且 16 从 M-F 图书馆，在二的相当或相同的事物报导了胚胎的性腺 cDNA 图书馆。涉及 epigenetic 和抄写规定的八基因的量的即时 PCR 分析证明在 CDK2AP1， SMARCE1， SAP18， SUDS3，和 PQBP1 的性之间的显著地不同的表示在性腺开发(E4 ) 出现在早阶段。把表示基因基于性差别的功能的比较，包括 ATP5A1W 的一些通常认为地重要的基因的角色， CDK2AP1， mitochondrial 抄本，等等被分析了。在结论，孤立的基因的描述将提供珍贵线索识别涉及鸡性区别和性腺开发的基因机制的潜在的候选人。

关 键 词：CHICKEN GONAD suppression subtractivehybridization sex differentiation 

分 类 号：Q78[生物学—分子生物学] Q959.468