银杏叶提取物对大鼠坐骨神经切断后HSP 70在脊髓与脊神经节中表达的影响  

Expression of heat shock proteins 70 in spinal cord and dorsal root ganglia after neurotomy of rat sciatic nerve and the effect of extract of ginkgo biloba

作　　者：骆渊城[1] 康皓[1] 李进[1] 陈燕花[1] 胡克力[1] 洪光祥[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院手外科,武汉 430022

出　　处：《中华手外科杂志》2009年第2期107-109,共3页Chinese Journal of Hand Surgery

基　　金：湖北省自然基金资助项目（编号：2004ABA192）

摘　　要：目的研究周围神经损伤后脊髓热休克蛋白（heat shock proteins，HSPs）的表达变化，及银杏叶提取物EGb 761对其影响，探讨银杏叶提取物对周围神经损伤的保护机制。方法取成年雄性SD大鼠144只，随机分成三组：对照组、坐骨神经切断组、坐骨神经切断＋银杏叶提取物干预组[术后每天用EGb 761（100mg·kg^-1·d^-1，溶于2ml SAL）灌胃，直到取材]，每组48只。术后6h、12h、1d,2d,4d、7d、14d、28d取L4-6。节段脊髓节段，采用免疫组织化学方法检测HSP70在脊髓前角及脊神经节中的表达。结果正常大鼠脊髓与神经节中均有少量HSP70表达，在坐骨神经切断后表达迅速增加，持续一段时间后又回到正常水平，用EGb761干预后，HSP70表达早期较未干预组表达低，后期表达增高，且表达持续时间长。结论银杏叶提取物EGb761可以增加神经损伤后HSP70表达，可能是银杏叶提取物保护神经，促进神经再生作用的机制。Objective To study the expression of heat shock protein 70 （HSP 70） in spinal cord and dorsal root ganglia after peripheral nerve injury and the influence of Ginkgo biloba extract EGb 761 on HSP 70 expression, and investigate the mechanism of the protective effect of EGb 761 after peripheral nerve injury in rats. Methods One hundred and forty-four male adult SD rats were randomly divided into three groups, sham operation group （n = 48）, sciatic nerve transection group （n = 48） and sciatic nerve transection and EGb 761 gastric perfusion group （ n = 48） （100 mg.kg^-1.d^-1 dissolved in 2 ml normal saline administered daily）. At 6 hours, 12 hours, 1 day, 2 days, 4 days, 7 days, 14 days and 28 days postoperatively, spinal cord of L4-L6 were harvested. Immunohistochemistry analysis was done to measure the expression of HSP 70 in anterior horns of L4-L6 spinal cord and dorsal root ganglia. Results In normal rats＇ spinal cord anterior horn and dorsal root ganglia, lISP 70 was expressed. After rat sciatic nerve transection, the expression of HSP 70 increased quickly. This elevated expression lasted for a period of time, and then returned to the normal level. After being intervened by EGb 761, HSP 70 expression during the early postoperative period was lower compared with the group not being intervened. At the later period, the expression increased and lasted for a longer time. Conclusion Ginkgo bfloba extract（EGb 761 ） can enhance the expression of HSP 70 after peripheral nerve injury. This could be the mechanism of the neuroprotective and neurotrophic effect of Ginkgo biloba extract.

关 键 词：神经再生 脊髓 神经节 脊 热休克蛋白质类 银杏叶提取物EGb 761 

分 类 号：R686[医药卫生—骨科学]