应用黑素细胞培养基在成年SD大鼠雪旺细胞培养中的实验研究  

Appfication of melanocyte growth medium in the culture of Schwann cells from adult SD rats： an experimental study

作　　者：章少华[1] 劳杰[1] 李岩峰[1] 顾玉东[1] 赵新[1] 李继峰[1] 

机构地区：[1]复旦大学附属华山医院手外科,上海200040

出　　处：《中华手外科杂志》2009年第2期110-112,共3页Chinese Journal of Hand Surgery

基　　金：国家973计划课题基金资助项目（2005CB522604）;上海市周围神经显微外科重点实验室课题基金资助项目（05DE22108）

摘　　要：目的寻找简单快速获得高纯度雪旺细胞的方法。方法取20只预变性成年SD大鼠坐骨神经，经分次酶消化后，分别使用DMEM／F12（A组）和黑素细胞培养基（B组）进行培养。冷喷射（cold jet）方式传代，S-100染色鉴定。结果采用黑素细胞培养基组的雪旺细胞纯度达到90％以上。结论使用黑素细胞培养基及冷喷射传代操作简单，可以在短期内获得足量高纯度的雪旺细胞。Objective To develop a simple and effective method to obtain highly purified Schwann cells. Methods After enzymatic digestion of Predegenerated sciatic nerves from 20 adult SD rats were with 1 week ion, the suspension were cultured in group A （DMEM/F12 medium） and group B （melanocyte growth medium）, cold jet as the passage method. S-100 stain for detection of Schwann cell purity. Results The cell yields and purity of group B was beyond 90%. Conclusion The combination of melanocyte growth medium and cold jet provides a convenient mean to obtain considerable Schwann cells with purity.

关 键 词：许旺细胞 细胞培养技术 黑素细胞培养基 

分 类 号：R686[医药卫生—骨科学]