临床患者粪便标本中艰难梭菌感染状况研究  

Research on Clostridium Difficile Infection in Clinic Patients Feces Specimen

作　　者：程颖[1] 卢金星[1] 鄢盛恺[2] 王靖[2] 李杰[2] 刘迎春[2] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206 [2]卫生部中日友好医院检验科,北京100029

出　　处：《中华医院感染学杂志》2009年第7期861-863,共3页Chinese Journal of Nosocomiology

基　　金：卫生部传染病重大专项(2008ZX10004-012)

摘　　要：目的探讨医院艰难梭菌带菌及感染状况,分析艰难梭菌相关性腹泻(CDAD)的临床特征,为临床提高艰难梭菌分离率以及采取积极有效的防控措施奠定基础。方法随机收集20例要求做粪便培养的患者标本,采用艰难梭菌毒素A/B检测试剂盒进行毒素检测,对标本进行厌氧培养与分离,并进行耐氧实验,用API 20A生化条进行确证。结果20例患者经艰难梭菌选择性培养基培养后,8株疑似菌落进行涂片镜检和耐氧实验,6株革兰阳性杆菌耐氧实验阳性,再经API 20A鉴定,4株为艰难梭菌,占所有患者的20%;毒素阳性1例,占5%。结论艰难梭菌的感染与某些内科基础性疾病的存在相关;水样的粪便标本更易于培养出艰难梭菌。OBJECTIVE This study was to investigate the carrier and infection of Clostridium difficile in clinic feces specimen, to analyze clinic characteristics, and to improve isolation rate and to provide basis on efficient prevention. METHODS C. difficile toxin A＆B kit and anaerobic culture was conducted in 20 cases with diarrhea. Colonies suspected to be C. difficile, on the basis of their macroscopic appearance and characteristic odor,oxygen tolerance experiment, were confirmed by their biochemical characteristics （API 20A, bioMerieux）. RESULTS After C. difficile selective culture, 8 suspected colonies from 20 feces specimen were conducted by feces smear and oxygen tolerance experiment. 6 of 8 was G^＋ rod bacteria with positive oxygen tolerance experiment. 4 stains of C. difficile were identified by API 20A, positive rate was 20% ; toxin detect was positive in 1 specimen （5%）. CONCLUSIONS Infection of C. difficile Is associated with the basic disease. Watery feces specimen was prone to culture positive.

关 键 词：艰难梭菌 艰难梭菌相关性腹泻 厌氧培养 

分 类 号：R181.32[医药卫生—流行病学]