慢性缺血性心力衰竭模型制备方法的对比研究  

Comparative study on methods for preparation of models of chronic ischemic heart failure

作　　者：张宁坤[1] 高连如[1] 许如意[1] 沈燕华[1] 陈宇[1] 李贤峰[1] 王丽华[1] 费宇行[1] 杨晔[1] 

机构地区：[1]海军总医院心血管内科,北京100037

出　　处：《中华老年心脑血管病杂志》2009年第4期285-287,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：首都医学发展基金(2003-2025)

摘　　要：目的探讨冷冻左心室前壁与结扎前降支两种方法诱导大鼠心肌梗死后制作慢性心力衰竭模型的对比研究。方法选择8～10周龄SD健康大鼠120只,随机分为冷冻组(50只),结扎组(50只)、假手术组(20只)。手术后4周行心电图、心功能检查,取心脏组织行TTC、HE染色并观察其死亡率。结果手术后4周冷冻组大鼠死亡率为32%低于结扎组48%。与假手术组比较,冷冻组、结扎组大鼠心电图显示为心肌梗死,LVEF、左心室短轴缩短率明显降低,左心室舒张末直径明显升高,差异有统计学意义(P<0.01),冷冻组和结扎组大鼠心肌切片、TTC染色可见100%梗死区;梗死区HE染色主要为瘢痕组织,假手术组未见心肌梗死。结论冷冻法诱导的心力衰竭模型在心功能、心肌梗死面积等方面等同于结扎法,且死亡率明显减低,冷冻左心室前壁诱导大鼠心肌梗死后慢性心力衰竭模型较结扎法更好。Objectives To compare preparation of the model of chronic heart failure after acute myocardial infarction by cryoinjury of anterior wall of left ventricle or ligation of anterior descending branch in rats. Methods 120 SD rats were randomized into eryoinjury （50）, ligation （50） and sham-operated groups（20）. ECG and heart function examination were performed 4 weeks after operation. Heart tissues were taken for TTC and HE staining and mortality of the rats was observed. Results Four weeks after operation, the mortality of cryoinjury group was 32%,which was lower than 48% in ligation group. Compared with sham-operated group,ECG showed myocardial infarction,obvious decrease in LVEF and shortening rate of short axis of left ventricle and obvious increase in end diastolic diameter of left ventricle in cryoinjury group and ligation group, the differences had statistical significance （P 〈 0.01）. TTC stained myocardium section of rats in cryoinjury group and ligation group showed 100% infarction area. Hematoxylin-eosin staining showed scar in myocardial infarction area, while myocardial infarction was not found in sham-operated group. Conclusions Heart function and myocardial infarction size of cryoinjury-induced model were equal to those in ligation-induced model. The mortality of cryoinjury model was lower than that of ligation model. The model induced by cryoinjury of left ventricle anterior wall is better than that induced by ligation of anterior descending branch.

关 键 词：心力衰竭 充血性 心肌梗塞 冷冻 结扎术 死亡率 模型 动物 死亡率 

分 类 号：R541.6[医药卫生—心血管疾病]