Effect of hydraulic retention time on the hydrogen yield and population of Clostridium in hydrogen fermentation of glucose  

作　　者：CHU Chunfeng EBIE Yoshitaka INAMORI Yuhei KONG Hainan 

机构地区：[1]School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China [2]National Institute for Environmental Studies, Tsukuba 305-8506, Japan [3]Faculty of Symbiotic Systems Science, Fukushima University, Fukushima 960-1296, Japan

出　　处：《Journal of Environmental Sciences》2009年第4期424-428,共5页环境科学学报（英文版）

摘　　要：The conversion of glucose to hydrogen was evaluated using continuous stirred tank reactor at pH 5.5 with various hydraulic retention times （HRT） at 30℃. Furthermore, the population dynamics of hydrogen-producing bacteria was surveyed by fluorescence in-situ hybridization using probe Clost IV targeting the genus Clostridium based on 16S rRNA. It was clear that positive correlation was observed between the cells quantified with probe Clost IV and hydrogen yield of the respective sludge. The numbers of hydrogen- producing bacteria were decreased gradually with increasing HRT, were 9.2 × 10^8, 8.2 × 10^8, 2.8 × 10^8, and 6.2 × 10^7 cell/mL at HRT 6, 8, 12, and 14 h, respectively. The hydrogen yield was 1.4-1.5 mol H2/mol glucose at the optimum HRT range 6-8 h. It is considered that the percentage of the hydrogen-producing bacteria to total bacteria is useful parameter for evaluation of hydrogen production process.The conversion of glucose to hydrogen was evaluated using continuous stirred tank reactor at pH 5.5 with various hydraulic retention times （HRT） at 30℃. Furthermore, the population dynamics of hydrogen-producing bacteria was surveyed by fluorescence in-situ hybridization using probe Clost IV targeting the genus Clostridium based on 16S rRNA. It was clear that positive correlation was observed between the cells quantified with probe Clost IV and hydrogen yield of the respective sludge. The numbers of hydrogen- producing bacteria were decreased gradually with increasing HRT, were 9.2 × 10^8, 8.2 × 10^8, 2.8 × 10^8, and 6.2 × 10^7 cell/mL at HRT 6, 8, 12, and 14 h, respectively. The hydrogen yield was 1.4-1.5 mol H2/mol glucose at the optimum HRT range 6-8 h. It is considered that the percentage of the hydrogen-producing bacteria to total bacteria is useful parameter for evaluation of hydrogen production process.

关 键 词：BIO-HYDROGEN fluorescence in-situ hybridization genus Clostridium 

分 类 号：X703[环境科学与工程—环境工程] Q943[生物学—植物学]