通络保肾复方对LPS刺激的肾小球系膜细胞增殖及其TGF-β_1表达的影响  

The Research on the effect of Tongluo Baoshen Prescription on GMCs proliferation and TGF-β_1 expression of GMCs stimulated by LPS

作　　者：宇汝翠 李建民 刘秀萍 

机构地区：[1]北京市中西医结合医院肾病糖尿病中心,北京100039

出　　处：《中国中医基础医学杂志》2009年第3期188-190,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：北京市中医药科技发展基金项目(2001)

摘　　要：目的:探讨通络保肾复方对肾小球系膜细胞(GMCs)增殖及肾小球系膜细胞转化生长因子-β1(TGF-β1)表达的影响。方法:将5%含药血清培养基加入体外培养的肾小球系膜细胞,分别于脂多糖(LPS)刺激肾小球系膜细胞12h、24h、48h、60h后,MTT方法检测各组肾小球系膜细胞的增殖情况,以及Western-blot方法检测LPS刺激48 h时肾小球系膜细胞TGF-β1的表达。结果:LPS刺激12h后肾小球系膜细胞开始增殖,48h达到高峰,60h后开始下降。通络保肾复方能够抑制肾小球系膜细胞的增殖,其中以通络保肾复方大剂量组和中剂量组效果显著,与LPS组比较差异显著(P<0.05)。同时通络保肾复方可以抑制48 h时由LPS刺激导致的肾小球系膜细胞TGF-β1的表达。结论:本研究结果提示,通络保肾复方可以抑制LPS诱导的肾小球系膜细胞的增殖及肾小球系膜细胞TGF-β1的表达,抑制细胞外基质的异常积聚和沉积,从而延缓肾小球硬化的进程。Objective：To investigate the effect of Tongluo Baoshen Prescription on proliferation of GMCs and expression of TGF-β_1 of GMCs stimulated by LPS.Methods：Preparation of serum contained 5% Tongluo Baoshen prescription was added in vitro GMCs.Investigate the proliferation of GMCs by MTT at 12 hours,24 hours,48hours and 60hours and the expression of TGF-β_1 of GMCs by Western-blot at 48hours after adding LPS.Results：The proliferation of GMCs started at 12hours and reached a peak at 48hours,then declined at 60hours.Tongluo Baoshen Prescription can reduce the proliferation of GMCs stimulated by LPS,in which the effect of high-dose group and middle-dose group was obvious compared with LPS group（P〈0.05）.The expression of TGF-β_1 of GMCs stimulated by LPS was inhibited at 48hours after adding Tongluo Baoshen Prescription.Conclusion：Tongluo Baoshen Prescription can reduce the proliferation of GMCs and the expression of TGF-β_1 of GMCs stimulated by LPS,and inhibit the accumulation of ECM,which is one of the mechanism of preventing and treating glomerular sclerosis.

关 键 词：通络保肾复方 脂多糖 TGFΒ-1 肾小球系膜细胞 肾小球硬化 

分 类 号：R285.5[医药卫生—中药学]