清纤方对肝纤维化大鼠肝脏胶原表达的影响  

Effects of Qing Xian Fang on Expression of Collagen in Livers of Rats with Liver Fibrosis

作　　者：张林军[1] 刘军[1] 马洪波[1] 白朝辉[1] 刘静[2] 常风云[3] 董尚普[4] 张明辉[2] 

机构地区：[1]河北体育学院医院,河北石家庄050041 [2]河北医科大学第一医院,河北石家庄050031 [3]河北医科大学中西医结合学院,河北石家庄050091 [4]河北医科大学中医学院,河北石家庄050091

出　　处：《中国中医基础医学杂志》2009年第3期194-196,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:探讨清纤方抗肝纤维化的治疗机制。方法:利用CCl4制备肝纤维化大鼠模型,分为正常组、模型组、清纤方组、秋水仙碱组。治疗8周后,用HE和Masson染色观察肝纤维化的形成,RT-PCR和免疫组化检测肝组织中Ⅰ、Ⅲ型胶原mRNA和蛋白的表达。结果:Ⅰ、Ⅲ型胶原mRNA和蛋白的表达在治疗组均明显减少。结论:清纤方对CCl4引起的肝纤维化治疗是有效的。Purpose：To explore the Qingxianfang therapy of anti liver fibrosis.Method：Prepare the model of fibrosis bandicoot liver by using CCl4.Models are divided into the normal group,model group,Anti-liver Fibrosis group and colchicines group.After a treatment of eight weeks,an observation is done by dyeing with HE and Masson,immunohistochemistry and detecting the expression of collagen mRNA type Ⅰ,type Ⅲ and protein with RT-PCR.Result：Collagen type Ⅰ,type Ⅲ and the expression of mRNA obviously decreased.Conclusion：Qingxianfang is able to reverse liver fibrosis caused by CCl_4.

关 键 词：肝纤维化 复合物 清纤方 Ⅰ、Ⅲ型胶原 

分 类 号：R285.5[医药卫生—中药学]