胡芦巴种质资源生物学特性的遗传多样性  

Genetic diversity in germplasm resources of Trigonella foenum-graecum based on biological characters

作　　者：刘萍[1] 张立杰[1] 马宏玮[1] 李应科[1] 康果[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《中草药》2009年第4期630-634,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30660017)

摘　　要：目的探讨不同胡芦巴种质资源生物学性状的遗传差异，初步分析其遗传多样性。方法以宁夏本地、国内其他省份及国外引进的32份胡芦巴种质资源为材料，在宁夏大学实验农场种植，调查其株高、结荚高度、主茎节数、分枝数、单株有效荚数等土要生物学性状，采用离差平方和法对欧氏距离进行聚类，分析其遗传多样性。结果在所研究的11个性状中，除荚长性状外，其余10个表型性状的方差分析均表现显著或极显著差异，变异系数最大的是总分枝数和单株有效荚数。成对种源的欧氏距离变化在1．11～9．49，平均值为4．01，单个种源欧氏距离（D）的平均值分布在2．94～6．54。在D=6．86处可以将32份种源划分成各具明显特点的3大类，与收集种源的地域基本一致。结论不同种源的胡芦巴间具有一定差异，多数国外种源与宁夏种源的遗传距离较远，国内其他省份种源与宁夏种源的遗传距离较近。Objective To analyze the genetic difference of biological characters on germplasm resources of fenugreek （Trigonella foenum-graecum） and to study the genetic diversity of them. Methods Thirty- two fenugreek germplasm resources from Ningxia, other provinces in China, and abroad were used to in vestigate the biological characters on plant height, pod formation height, nodes per main stem, branches number, effective pod per plant and so on. The tested materials were planted in the farm of Ningxia University. The genetic diversity based on the phenotype was analyzed by Word＇s minimum variance method. Results The variance analyses of ten biological characters were of significance or the most significance except pod length. The branches number and effective pod per plant possessed the largest coefficient of variance （CV）. The pairwise Euclidean distance of the tested materials ranged from 1.11 to 9.49, averaged 4. 01. Mean Euclidean distance of single tested material ranged from 2.94 to 6.54. The testedgermplasm resources could be divided into three groups in D=6.86, which coincided with the district of collecting germplasm resources on the whole. Conclusion There is a little genetic difference in various fenugreek germplasm resources. Compared with Euclidean distance of germplasm resources from Ningxia, the Euclidean distance of most from abroad is far away from that of other provinces in China.

关 键 词：胡芦巴 种质资源 生物学性状 聚类分析 遗传多样性 

分 类 号：R282.2[医药卫生—中药学]