罗布麻微生物脱胶工艺优化  

Optimizing microorganism degumming process of Apocynum

作　　者：薛卫巍[1,2] 翟秋梅[1] 薛永常[1] 郑来久[2] 

机构地区：[1]大连工业大学发酵工程重点实验室,辽宁大连116034 [2]大连工业大学纺织工程重点实验室,辽宁大连116034

出　　处：《纺织学报》2009年第4期80-84,共5页Journal of Textile Research

基　　金：罗布麻生物酶脱胶及其生态绿色产品开发项目(2006R13);辽宁省纺织材料绿色加工技术创新团队项目(2006T028)

摘　　要：为了提高枯草芽胞杆菌对罗布麻的脱胶效率,根据菌种生长与酶催化反应所需温度的差异,将罗布麻生物脱胶过程分为2个阶段,并对脱胶工艺进行优化。试验结果表明:在磷酸氢二钾添加量为0.8%、浴比为1∶25、枯草芽胞杆菌接种量为0.5%的条件下,在37℃脱胶18 h,再升温至45℃脱胶0.5 h,取得了良好的脱胶效果,罗布麻脱胶后的残胶率为10.84%,与未进行后阶段升温脱胶所得的残胶率相比降低了4.95%。Based on the difference of temperature needed for strain growth and enzymic catalytic reaction, the process of biological degumming using Bacillus subtilis was divided into two stages for good retting efficiency. The degumming conditions were optimized as follows： the amount of K2 HPO4, 0.8 % ; the proportion between bast and water, 1 ： 25 ; the inoculation amount, 0.5 % ; and under such conditions, degumming was carried out at 37 ℃ for 18 hours, and then raised the temperature to 45 ℃ and held for 0.5 hours. Good degumming result was obtained by this process with the residual gum eontent of 10.84%, which was decreased by 4.95% when ompared with that without raising the temperature in the latter stage.

关 键 词：罗布麻 生物脱胶 枯草芽胞杆菌 工艺优化 

分 类 号：TS192.552[轻工技术与工程—纺织化学与染整工程]