多次LDR对12周糖尿病大鼠脾细胞凋亡及免疫的干预作用  

Interventional effect of multiple LDR on splenocyte apoptosis and immunity on twelve-week diabetic rats

作　　者：李艳博[1] 郭彩霞[2] 王志成[1] 李鹏武[1] 郭伟[1] 赵红光[1] 刘扬[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]吉林大学公共卫生学院卫生毒理学教研室,长春130021

出　　处：《辐射研究与辐射工艺学报》2009年第2期101-105,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(39870312)资助

摘　　要：观察了多次低剂量辐射(Low dose radiation,LDR)对12周糖尿病(Diabetes mellitus,DM)大鼠脾细胞凋亡、免疫因子和淋巴细胞亚群的影响。将实验动物分为对照组、单纯DM组和DM+LDR组,照射剂量分别为25、50和75mGy,共照射15次。照射结束后8周末采用流式细胞术(Flowcytometry,FCM)检测脾细胞中CD4+、CD8+T细胞和TCRαβ百分数的变化,酶联免疫吸附法(Enzyme linked immunosorbert assay,ELISA)检测血清和脾细胞培养上清IL-2含量的变化,以FCM和TdT介导的dUTP缺口末端标记技术(Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling,TUNEL)检测细胞凋亡。与对照组相比,DM和DM+LDR各组体重均下降,DM组尤为明显;DM+LDR组大鼠的血糖水平有升高,但低于DM组;DM+LDR各组TCRαβ和CD4+T细胞百分数、血清和脾细胞培养上清IL-2含量和脾细胞凋亡均升高。但与DM组相比,DM+LDR各组TCRαβ、CD4+和CD8+T细胞百分数及脾细胞凋亡均降低,而IL-2含量和CD4+/CD8+T细胞比值均升高。研究表明,多次低剂量照射能够适当调节以减弱DM所造成的大鼠体重减轻和血糖升高现象,纠正淋巴细胞亚群和免疫因子失衡,降低DM所致脾细胞凋亡,从而达到保护机体的作用。In order to explore the effect of multiple low dose irradiation （LDR） on the apoptosis of splenocytes, immune factors and lymphocyte subgroups in twelve-week diabetes mellitus （DM）, the experiment was carded out with control, DM and DM＋LDR groups. The irradiation dose every time was 25, 50 and 75 mGy respectively, and the irradiated times were 15. At the eighth weekend after the DM rats were irradiated, the percentages of CD4^＋, CD8^＋ T lymphocytes and TCR αβ were detected by flowcytometry （FCM）. The content of IL-2 in both serum and supernatant of cultured splenocytes were detected by ELISA. And the apoptofic rate of splenocytes was detected by FCM and TUNEL respectively. The result shows that as compared with that in the control, the body weight （BW） decreases both in the DM and the DM ＋ LDR groups, particularly in the DM group. The blood glucose （BG） level in the DM＋LDR groups is higher than that in the control, but lower than that in the DM group. As compared with those in the control, the percentages of TCR αβ and CD4^＋ T cells, the content of IL-2 in serum and supernatant of cultured splenocytes, and the apoptotic rate of splenocytes in DM ＋ LDR groups increase significantly. However, as compared with those in the DM group, the percentages of TCR αβ, CD4^＋ and CD8^＋ T cells and the splenocyte apoptofic rates in the DM＋LDR groups decrease while the content of IL-2 and the ratio of CD4^＋/CD8^＋ T cells increase. It is obvious that the multiple LDR could regulate and weaken the loss of BW and increase of BG caused by DM, correct the imbalance of lymphocyte subgroups and immune factors, decrease the increment of splenocyte apoptosis resulted from DM. Multiple LDR could result in body protection.

关 键 词：低剂量辐射 糖尿病 淋巴细胞 脾脏 细胞凋亡 IL-2 

分 类 号：R587.1[医药卫生—内分泌] R852.7[医药卫生—内科学]