UVC诱导的枯草芽孢杆菌DNA双链断裂及影响因素探讨  

The studies on DNA double-strand breaks of Bacillus subtilis irradiated by ultraviolet-C

作　　者：周莉薇[1] 陈晓明[1] 张建国[1] 柳芳[1] 张根发[2] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]北京师范大学生命科学学院,北京100875

出　　处：《辐射研究与辐射工艺学报》2009年第2期106-110,共5页Journal of Radiation Research and Radiation Processing

基　　金：西南科技大学核废物与环境安全国防重点学科实验室基金项目(07JGZB07);中国工程物理研究院预研基金(052056)资助

摘　　要：为探讨短波紫外线(Ultraviolet C,UVC)对枯草芽孢杆菌基因组DNA的损伤效应,探讨研究方法和材料对结果的影响,以枯草芽孢杆菌为研究材料,分别对其菌体样品及DNA样品进行不同剂量的UVC辐照,采用8h、16h、24h脉冲场凝胶电泳分离DNA片段。结果发现,16h脉冲场凝胶电泳最能反映DNA的双链断裂程度。对16h电泳图进行数据分析发现,随UVC辐照剂量的增大DNA释放百分比递增;菌体样品在辐照剂量17.8J/cm2处其DNA双链断裂产额最大,而DNA样品的最大双链断裂产额出现在辐照剂量为72.7J/cm2处;另外,同辐照剂量下菌体样品的释放百分比和菌体DNA双链断裂产额均高于DNA样品。结果表明,UVC诱导的枯草芽孢杆菌DNA双链断裂程度与辐照剂量及辐照样品密切相关。In order to investigate the DNA damage exposed to ultraviolet-C（UVC） and the effect both of detecting method and sample types on the result, the Bacillus subtilis and general DNA were used as the model samples irradiated with different doses of UVC respectively. Subsequently, DNA fragments were analyzed by 8 h, 16 h, and 24 h pulsed-field gel electrophoresis （PFGE）. The result has been revealed that the 16 h PFGE could reflect the DNA double strand break （DSB） more exactly. With the analysis of 16 h PFGE map it has been found that the percent of DNA release increases gradually with the increment of radiation doses. What＇s more, the most DSB appear at the dose of 17.8 J/cm^2 in the thallic samples and at the dose of 72.7 J/cm^2 in the DNA samples. The DSB is more significant in the thalli than in general DNA. The results indicate that the DSB level induced by UVC is related with irradiation doses as well as sample types.

关 键 词：UVC辐照 枯草芽孢杆菌 DNA双链断裂 脉冲场凝胶电泳 

分 类 号：Q345.24[生物学—遗传学] Q527.4