二妙丸多指标化学成分质量研究  

Quality study of multi-chemical compositions in Ermiao Pills

作　　者：张杨[1] 张振秋[1] 王海波[1] 王静[1] 张朔[1] 李可强 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药研究所,辽宁沈阳110161

出　　处：《中成药》2009年第4期562-566,共5页Chinese Traditional Patent Medicine

摘　　要：目的:建立二妙丸(苍术,黄柏)挥发油中β-桉叶醇含量的气相色谱测定法;对二妙丸单煎合并与合煎的挥发油及提取干膏进行多指标化学成分的测定和比较。方法:以丹皮酚为内标物,采用内标法和程序升温的气相色谱条件对二妙丸单煎与合煎的挥发油中的β-桉叶醇含量进行测定和比较;采用高效液相色谱法对二妙丸中的小檗碱、巴马汀、药根碱成分进行含量测定和比较。结果:二妙丸合煎的挥发油中的β-桉叶醇含量比单煎中的含量略高,分别为73.34 mg/g和70.04 mg/g;但从图谱的峰数和保留时间进行比较,结果无明显差别。单煎合并的小檗碱、巴马汀、药根碱的含量明显高于合煎的含量。结论:确立二妙丸挥发油中β-桉叶醇含量测定的方法。并且说明苍术、黄柏配伍,彼此相互影响。AIM： To establish methods of determing β-eudesmol in atractylis oil from Ermiao Pills （Rhizoma Atractylodis and Cortex Phellodendri Chinensis） by gas chromatography and determing and comparing the chemical compositions between atractylis oil and poultice of Ermiao Pills by decocting separately and together. METHODS ： β-eudesmol content in Ermiao Pills decocting separtely and together was determined and compared by GC using paeonol as the internal standard substance, and contents of berberine, palmatine, jatrorrhizine in Ermiao Pills decocting separately and together were determined and compared by HPLC. RESULTS： The β-eudesmol content in the volatile oil of decoction together was more than that of the decoction separately. But there was no remarkable difference. Berberine, palmatine, jatrorrhizine contents of decoction separately were more than that of the together. CONCLUSION： The method of determing β-eudesmol in Ermiao Pills is established. And it has mutual effect in compatibility with Cortex Phellodendri Chinensis and Rhizoma Atractylodis.

关 键 词：二妙丸 单煎 合煎 Β-桉叶醇 

分 类 号：R927.2[医药卫生—药学]