结缕草属植物耐盐性SRAP分子标记研究  

Identification of SRAP molecular markers linked to salt tolerance in Zoysia grasses

作　　者：陈宣[1] 郭海林[1] 薛丹丹[2] 郑轶琦[2] 刘建秀[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]南京农业大学,江苏南京210095

出　　处：《草业学报》2009年第2期66-75,共10页Acta Prataculturae Sinica

基　　金：江苏省高技术研究项目(BG2006320);国家青年科学基金项目(30800759)资助

摘　　要：本研究应用混合集群分析法,通过建立结缕草属植物耐盐性两极端类型材料DNA池对400对SRAP引物组合进行筛选,得到111对多态性引物组合,再以日本结缕草耐盐两极端材料DNA对111对具有特异带的引物组合进行进一步筛选,从中获得22对多态性引物组合,最后用群体各单株对具有耐盐特异带的引物组合进行验证,获得了7个与结缕草属植物耐盐性紧密相关的SRAP分子标记,依据扩增条带的大小分别命名为:Me9-Em4260、Me9-Em18720、Me13-Em18500、Me5-Em20180、Me14-Em7220、Me6-Em17600、Me2-Em18260,以上筛选得到的SRAP分子标记将为深入开展结缕草属植物分子标记辅助育种及耐盐基因克隆奠定基础。In this paper, bulked segregant analysis was used to identify SRAP molecular markers linked to salt tolerance in Zoysia grasses using two salt-tolerant, extreme types of DNA pools. From 400 pairs of SRAP primer combinations, 111 were found with polymorphic bands. These were further screened using two extreme salt-tolerant samples of Zoysia japonica and 22 pairs of polymorphism primer combinations were selected. Finally, the primer combinations with specific bands were verified using salt-tolerant individuals and 7 SRAP molecular markers closely linked to salt tolerance were obtained. Based on the size of the amplified bands they were named： Me9-Em4260 Me9-Em18720 Me13-Em18500,5-Em20180 Me14-Em7220 Me6-Em17550 Me2- Em18260 This research will accelerate zoysia grass marker-assisted selection breeding and lay the foundation for salt-tolerant gene cloning.

关 键 词：结缕草 耐盐性 SRAP分子标记 

分 类 号：S688.4[农业科学—观赏园艺]