曙红B及变色酸与核酸作用的光谱学性质研究  

The interactions of eosin B and chromotropic acid with DNA

作　　者：王海燕[1] 栾尼娜[1] 宋玉民[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2009年第4期491-495,共5页Chemical Research and Application

基　　金：甘肃省自然科学基金项目(0710JRZA108);甘肃省高分子材料重点实验室项目资助

摘　　要：利用荧光光谱法、紫外-可见吸收光谱法、圆二色谱法、凝胶电泳法对曙红B及变色酸与核酸的作用机理进行了探讨。当ct-DNA浓度增加时,曙红B和变色酸的荧光光谱呈荧光增强趋势;紫外-可见光谱呈下降趋势;并使ct-DNA圆二色谱正负峰吸收强度呈现一定的降低;琼脂糖凝胶电泳实验中,二者在泳道中与DNA作用发出的荧光在紫外灯下皆清晰可见。并将所得结果与溴化乙锭-DNA体系性质进行对比,为寻找高效低毒的DNA分子荧光探针提供了实验基础。The interactions between Eosin B and DNA, chromotropic acid and DNA are studied by fluorescence , UV-absorption , circular dichroism spectral and agarose gel electrophoresis methods. When DNA exists, the fluorescence intensity of Eosin B and chromotropic acid put up a direction of increasing. The absorbance of UV spectra both present a hypochromic effect when DNA concentration rise. The CD spectra of DNA has a great degrcssive both in negative and positive spectra with Eosin B and chromotropic acid added. In the agarose gel electrophoresis, the luminescence intensity of Eosin B -DNA system and chromotropic acid-DNA system can be seen clearly. By comparing the above research results with EB-DNA system, we get a conclusion that Eosin B, Chromotropic acid and ethldium bromide have the same modes when interaction with DNA. This study afford basal experimentation for searching more sensitive and less poisonous DNA fluorescence probe.

关 键 词：曙红B 变色酸 溴化乙锭 核酸 光谱学性质 

分 类 号：O641.3[理学—物理化学]