云南白药对实验性糖尿病牙周炎大鼠的临床及代谢组学研究  

The clinical and metabonomics study of Yunnan Baiyao on experimental diabetic periodontitis rats

作　　者：和红兵[1,2] 石先哲[1] 陈静[1] 王文昭[1] 许国旺[1] 

机构地区：[1]中国科学院大连化学物理研究所代谢组学研究中心,大连116023 [2]昆明医学院口腔内科教研室,昆明650031

出　　处：《世界科学技术-中医药现代化》2009年第1期195-200,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：云南省应用基础研究基金(2007c218m):云南白药对实验性糖尿病牙周炎大鼠代谢指纹分析;负责人:和红兵

摘　　要：通过云南白药对糖尿病牙周炎动物模型影响的临床和代谢物分析,探讨云南白药对糖尿病牙周炎的影响。方法:建立实验性糖尿病牙周炎动物模型,观察应用云南白药后大鼠的临床及组织学变化。基于超高效液相色谱-飞行时间质谱(UPLC-TOFMS)测定云南白药给药后大鼠尿液代谢物变化。结果:(1)灌药后2周至6周云南白药组(BY)牙周探诊深度(PD)逐渐下降,组间两两比较均有统计学意义(P<0.05),在6周时与模型组(DP)组相比BY组的PD及牙槽骨丧失(ABL)出现明显下降(P<0.05),组织学观察也在6周时发现BY组有牙槽骨修复及新骨形成。(2)结合PLS-DA发现BY组变化较明显,在2周时偏离最远,4周后逐渐回归,6周时与0周时基本重合,与糖尿病牙周炎大鼠PD及ABL变化情况相一致。初步表明云南白药能延缓糖尿病牙周炎病程的发展。Objective： The goal of this study was to investigate the effect of Chinese traditional medicine Yunnan Baiyao on the diabetic periodontitis by clinical and metabonomic analysis. Methods： diabetic periodontitis Sprague Dawley rats model were set up. Two, four and six weeks after the use of Yunnan Baiyao the clinical assessment and histological observation were made including probing depth （PD） and alveolar bone loss （ABL）. Results： 1） The sequence of mean probing depth in Yunnan Baiyao group was 2 wk 〉 4 wk 〉 6 wk （P 〈 0.05）, intergroup comparison demonstrated that both mean PD and mean ABL of Yunnan Baiyao group were decreased compare with those of diabetic periodontitis group （P 〈 0.05） at 6 wk. 2） Based on the urinary metabolite profiles investigated using ultra high performance liquid chromatography-time of flight mass spectrometry technique, it was found that there is metabonome difference between Yunnan Baiyao group and healthy group in 2wk, and tend back to healthy group at 4wk , in 6wk Yunnan Baiyao group shows overlap with healthy group. Taken together, it is safe to say that Yunnan Baiyao could retard the evolution of diabetic periodontitis.

关 键 词：云南白药 糖尿病牙周炎 超高效液相色谱 飞行时间质谱 牙槽骨 

分 类 号：R587.1[医药卫生—内分泌] R286[医药卫生—内科学]