石楠抗寒基因AmGS高效转化体系的研究  

STUDY ON THE HIGHLY EFFECTIVE TRANSFORMATION SYSTEM OF COLD-TOLERANT GENE AMGS TRANSFORMED INTO CHINESE PHOTINIA

作　　者：刘静[1] 王长宪[1] 王斌[2] 孙仲序[3] 刘杰[4] 黄艳艳[1] 赵进红[1] 张虹[1] 

机构地区：[1]泰安市泰山林业科学研究院,泰安271000 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]山东农业大学园艺学院,泰安271018 [4]青岛科技大学生物系,青岛266042

出　　处：《山东农业大学学报（自然科学版）》2009年第2期191-194,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：吉林省科技厅资助项目(200705C05);通化师范学院自然科学基金资助项目(XS070077)

摘　　要：本研究以石楠为受体材料，利用农杆菌浸染方法开展沙冬青抗寒基因AmGS转化试验，通过对不同的受体材料浸染部位、菌液浓度、乙酰丁香酮和浸染时间对转化材料的死亡率％、分化率％、分化芽数（个）、芽苗长度（cm）以及芽苗颜色的分析，得出最佳转化方法为：采用茎尖为转化材料，菌液浓度为2／3～1／2原液，浸染时间为10min，添加乙酰丁香酮浓度为25—30mg／L。其中浸染部位是影响基因高效转化的最主要因素，菌液浓度、乙酰丁香酮和浸染时间的作用次之，经在50mg／L浓度的卡那霉素培养基筛选培养，获得一批的抗性植株，经PER检测获得6株转化株系。By means of Agrobacterium contamination, cold - tolerant gene AmGs, absorbed form Ammopiptanthus mongolicus, was transformed into Chinese photinia. We made an investigation what different contamination position, Agrobacterium concentration, aeetosyringone and contamination time of the acceptor material affected the transformant death rate, differentiation rate, number of differential buds, buds length and buds color. Then we got the best transformation method that Agrobacterium was 2/3to 1/2 as dense as original liquid, acceptors were contaminated for 15 minutes and acetosyringone was 25 mg/L to 30 mg/L when stem apexes were trans- formed. Contamination position was the major factor of effects on the gene advanced transformation. The effects of bacterial concentration and contamination time were minor factors. A group resistant plants wene obtained through with the ouhure medium contains 50 mg/L kanamycin, then six transferplants can he got by PCR examination.

关 键 词：石楠农杆菌浸染 抗寒基因 AmGS 高效转化 

分 类 号：S685.12[农业科学—观赏园艺]