L-色氨酸的分子印迹膜电化学传感器的研制与应用  

ELECTROCHEMICAL SYNTHESIS OF L-TRYOTOPHAN MOLECULAR IMPRINTING FILM SENSOR AND ITS APPLICATION

作　　者：马永飞[1] 曲祥金[1] 殷焕顺[1] 杜红霞[1] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2009年第2期254-258,共5页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家自然科学基金项目(No.20775044);山东省自然科学基金项目(No.Y2006B20)

摘　　要：以2-巯基苯并咪唑为聚合单体，L-色氨酸为模板分子，在pH值为9．37的条件下，采用循环伏安法直接在金电极表面聚合制得L-色氨酸的分子印迹膜，作为电化学传感器，用以检测L-色氨酸。由于L-色氨酸本身无电化学活性，实验中以铁氰化钾作为探针分子，采用恒电位计时安培法对L-色氨酸进行快速检测，响应在100s内达到稳定，并且有良好的选择识别性和重现性。测定的线性范围为3．10×10^-5～8．83×10^-4mol／L。本文还测定了2-巯基苯并咪唑与L-色氨酸络合物的结合常数，讨论了该传感器的最佳检测条件。The L - tryptophan molecular imprinting film sensor, which select 2 - mercaptobenzimidazole and L - tryptophan as monomer and template molecule, respectively, was prepared directly on the surface of gold electrode by cyclic vohammetry method. Because of L - tryptophan has no electrical activity, the potassium ferricya- nide was used as a molecular probe to detect L -tryptophan by constant potential amperometry. The response a- chieve stable in 100 s. The sensor exhibited good selectivity and sensitivity to L - tryptophan. The linear range for the determination of L -tryptophan is from 3.10 × 10^-5 to 8.83 × 10^-4mol/L,the binding constant of 2 - mercaptobenzimidazole and L - tryptophan were detected, and the best detection condition of this sensor were discussed.

关 键 词：电聚合 分子印迹 L-色氨酸 2-巯基苯并咪唑 

分 类 号：O636[理学—高分子化学]