ICAM-1在实验性自身免疫性脑脊髓炎发病机制中的作用  

The investigation to the effect of ICAM-1 in the nosogenesis of experimental autoimmune encephalomyelitis

作　　者：赵亚朴[1] 王红[1] 刘慧敏[1] 王秀卿[1] 黄庆军[2] 

机构地区：[1]白求恩军医学院,石家庄050081 [2]汕头大学精神卫生中心,汕头515063

出　　处：《白求恩军医学院学报》2009年第2期67-69,共3页Journal of Bethune Military Medical College

摘　　要：目的检测实验性自身免疫性脑脊髓炎(EAE)大鼠血脑屏障中血管内皮细胞细胞间粘附分子-1(ICAM-1)的表达,并分析其表达与EAE发病的关系。方法取EAE组和对照组大鼠的脑组织,石蜡切片,免疫组化染色,图像扫描系统观测,图像分析软件进行灰度分析。结果EAE组大鼠脑血管内皮细胞上ICAM-1的表达明显深于对照组。实验组的灰度值为141.47±6.88,而对照组为166.24±7.24,两组差异非常显著(P<0.01)。结论大鼠EAE时脑血管内皮细胞ICAM-1的表达上调,提示ICAM-1是与EAE发生相关的重要粘附分子。Objective To investigate the relationship between the expression of ICAM- 1 in the blood-brain barrier and the experimental autoimmune encephalomyelitis（EAE）. Methods Brain of EAE as well as control animals were taken and embedded in paraffin. And the sections were stained by immunohistochemical method. Then the sections were observed by Nikon image scanning system arid measured the gray value by IPP image analysis software. Results The expression of ICAM-1 in the epithelial cell of endangium of EAE rat＇ s brain was higher than that in control. Conclusion The increase of the expression of ICAM-1 in the epithelial cell of endangium of EAE rat＇ s brain indicated that ICAM-1 was a necessary adhesion molecule in the developmental process of EAE.

关 键 词：免疫组织化学法 ICAM-1 血脑屏障 实验性自身免疫性脑脊髓炎 

分 类 号：R392.11[医药卫生—免疫学]