生理性雌激素对骨髓源性内皮祖细胞迁移功能的影响  

Effects of physiological estrogen on the migratory capacity of bone marrow-derived endothelial progenitor cells

作　　者：李海清[1] 赵强[1] 孙晓宁[1] 魏来[1] 叶晓峰[1] 

机构地区：[1]上海市复旦大学附属中山医院心外科,200032

出　　处：《中国分子心脏病学杂志》2009年第2期79-83,共5页Molecular Cardiology of China

基　　金：上海市重点学科建设资助项目(B116)

摘　　要：目的研究生理性雌激素对骨髓源性内皮祖细胞(BM-EPCs)迁移功能的影响及其机制。方法雌性BALB/C小鼠随机分成卵巢切除组、假手术组和正常组。ELISA法检测各组血清雌激素浓度。取胫骨和股骨骨髓,培养、鉴定BM-EPCs。Transwell小室检测各组BM-EPCs经或未经CXCR4抑制剂AMD3100处理后向基质细胞衍生因子-1α(SDF-1α)的迁移功能。RT-PCR和流式细胞术检测各组BM-EPCs CXCR4的表达。结果卵巢切除组血清雌激素浓度明显低于假手术组和正常组(P<0.01)。卵巢切除组BM-EPCs向SDF-1α迁移的数量明显低于假手术组和正常组(P<0.01)。AMD3100明显抑制BM-EPCs向SDF-1α的迁移功能。卵巢切除组BM-EPCs CXCR4的表达明显低于假手术组和正常组(P<0.01)。结论生理性雌激素通过调节BM-EPCs CXCR4的表达而增强其迁移功能。Objective To investigate the effects and mechanisms of physiological estrogen on the migratory capacity of bone marrow-derived endothelial progenitor cells （BM-EPCs）. Methods Six weeks female BALB/C mice were randomly divided into ovarieetomized group, sham operative group and normal group. ELISA was taken to measure serum levels of estrogen in each group 1 and 4 weeks after observation. 4 weeks later, we cultured and identified BM-EPCs, assessed migratory capacity of BM-EPCs of each group toward stromal cell-derived faetor-1α （SDF-1α） with or without treatment of CXCR4 inhibitor AMD3100 by Transwell chamber, and detected expression of CXCR4 by RT-PCR and FACS. Results First, serum levels of estrogen in the ovariectomized group 1 and 4 weeks after ovarieetomy were both significantly lower than sham operative group and normal group （P 〈 0.01 ）. Second, the number of BM-EPCs migrated toward SDF-1α in ovariectomized group was significantly decreased than in shame operative group and in normal group, respectively （P 〈0.01 ）. After administration of AMD3100, the number of BM-EPCs migrated to SDF-1α in each group was significantly reduced （P 〈 0.01 ）, but there was no difference between them （P 〉 0.05 ）. Finally, CXCR4 expression of BM-EPCs from ovariectomized group significantly decreased than sham opera- tive group and normal group （ P 〈 0.01 ）. Conclusion Physiological estrogen improves migratory capacity of BM-EPCs by up-regulating CXCR4 expression.

关 键 词：雌激素 内皮祖细胞 迁移 CXCR4 基质细胞衍生因子-1Α 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]