骨髓间充质干细胞和骨髓单个核干细胞移植治疗大鼠心肌梗死的对比研究  

A comparison study of transplantation of bone marrow mesenchymal stem cells and bone marrow mononuclear stem cells for myocardial repair in rats

作　　者：乔志钰[1] 谢进生[1] 杨传瑞[1] 白涛[1] 黄信生[1] 

机构地区：[1]首都医科大学附属北京安贞医院心外科,北京100029

出　　处：《中国医药》2009年第4期241-243,共3页China Medicine

基　　金：北京市科技新星计划（954813700）

摘　　要：目的观察髓间充质干细胞（MSCs）和骨髓单个核干细胞（BMMNCs）移植对急性心肌梗死后大鼠左心功能的影响及存活情况，比较两种干细胞移植的优劣性。方法雌性Lewis大鼠45只按随机抽签法分3组：对照组、MSCs组、BMMNCs组。结扎前降支制备心肌梗死模型，MSCs组和BMMNCs组均注射等数量（2×10^6）雄性MSCs和BMMNCs，对照组注射等体积的培养基。4周后超声检测左心功能、左心室前壁厚度，免疫原位杂交检测雄性鉴别基因sry片段的表达。结果MSCs组和BMMNCs组与对照组相比：左心室射血分数分别增加22．57％、24．09％（P=0．000），MSCs组和BMMNCs组之间比较，差异无统计学意义（P〉0．05）；左心室前壁厚度增加（2．6±0．5）mm和（2．5±0．3）mm对（1．8±0．4）mm（P=0．000）；免疫原位杂交显示MSCs组与BMMNCs组sry表达阳性，对照组阴性。阳性细胞的数量相比较，BMMNCs组高于MSCs组（66．50±6．59比46．67±4．64，P=0．000）。结论MSCs和BMMNCs移植均能改善大鼠急性心肌梗死后左心功能，减轻左心室重构；移植4周后在受体心肌内存活干细胞数量BMMNCs优于MSCs。Objective To compare the outcomes of mononuclear stem （BMMNCs） and mesenchymal stem （MSCs） cell therapy for myoeardium infarction in rats. Methods In 45 female Lewis rats, myocardial infarction was induced by direct ligation of LAD. MSCS （2 × 10^6 ） and BMMNCs （2 × 10^6 ） harvested from male rats were injected directly into the infarcted myocardium in the infarct zone. Four weeks after therapy echocardiography was performed and animals were sacrificed for further analysis. The survival outcomes of MSCs and BMMNCs were detected with expression of sry gene of Y chromosome by using fluorescence in situ hybridization （FISH）. Results Four weeks later, left ventricular function was significantly improved in MSCs and BMMNCs groups compared with the control group （LVEF： 54.9 ± 3.4% , 56.5 ± 3.8% vs. 32.4 ± 4.0%, P = 0.000）. The thickness of LV anterior wall significantly increased in MSCs and BMMNCs groups compared with the control group （2.6 ±0.5 mm and 2.5 ± 0.3 mm vs. 1.8 ± 0.4 mm , P = 0. 000）. FISH showed that sry positive cells were observed in myocardium in MSCs and BMMNCs groups but not found in the control group. At the same time the number of sry positive cells in BMMNCs was significantly higher than that in MSCs （66.50 ± 6.59 vs. 46.67 ± 4.64, P = 0. 000）. Conclusion The research shows that both MSCs and BMMNCs from bone marrow can repair the acute infarcted myocardium. The myocardial survival of BMMNCs is superior to that of MSCs.

关 键 词：心肌梗死 骨髓间充质干细胞 骨髓单个核细胞 移植 

分 类 号：R542.22[医药卫生—心血管疾病]