基于纳米铂黑修饰的快速检测用乳酸生物传感器研究  

Development of Amperometric Lactate Biosensor Modified with Pt-black Nanoparticles for Rapid Assay

作　　者：刘春秀[1,2] 刘红敏[1,2] 杨庆德[1,2] 田青[1,2] 蔡新霞[1,2] 

机构地区：[1]中国科学院电子学研究所传感技术联合国家重点实验室,北京100080 [2]中国科学院研究生院,北京100080

出　　处：《分析化学》2009年第4期624-628,共5页Chinese Journal of Analytical Chemistry

基　　金：国家863计划项目(No.2007AA04Z326);国家自然科学基金项目(No.60801032);中科院创新项目(No.KGCX2-YW-111-2);中科院电子所创新项目(Nos.08SQCX-9251790151,07QNCX-9240)资助课题

摘　　要：制备了一种可用于运动员血清样品乳酸快速检测的L-乳酸传感器。这种便携式平面电化学生物传感器采用金薄膜两电极系统;先后修饰纳米铂黑粒子层和铁氰化钾媒介体。铂黑纳米粒子沉积于金电极表面以提高传感器的灵敏度和稳定性,然后将乳酸氧化酶(LOD,E.C.1.1.3.2)和相关试剂固定在工作电极表面,铁氰化钾作为媒介体用以提高电极表面电子传递能力,并将工作电压降低为0.2V。通过优化铂黑颗粒的沉积、乳酸氧化酶的浓度、铁氰化钾的浓度、添加剂的成分和浓度等条件,将传感器的检测范围扩展至1～20mmol/L乳酸,检测灵敏度提高到1.43μA·L/mmol,检测时间为50s。生物传感器的批间r为0.0549;生物传感器经室温储存1年后仍可保持90%的活性。这种传感器成功地用于无稀释乳酸血清样品的快速检测,结合便携式检测仪(YT2005-1乳酸测试仪)将在快速诊断领域具有很好的应用前景。For point-of-care determination, a disposable biosensor for the analysis of L-lactate in athletes＇ serum samples had been developed and optimized. The planar electrochemical biosensor was fabricated with gold thin-film two-electrode system, and modified with platinum-black nano-particles and ferricyanide mediator. Platinum-black nano-particles were deposited on the electrodes to improve the sensitivity and stability. Lactate oxidase（LOD, E. C. 1. 1.3.2） was immobilized on working electrodes with ferricyanide as mediator improving electron transfer ability, by lowering the work potential to ＋ 0.2 V. Optimizations of deposition of platinum-black nano-particles, concentration of LOD, concentration of ferricyanide have resulted in the development of a lactate electrode with a wide linear range of 1 - 20 mmol/L lactate, a high sensitivity of 1.43 μA · L/mmol, a fast detection time of 50 s, and a RSD among batches of 5.5%. The activity of biosensor held above 90% after one year storage at room temperature. The biosensor was successfully applied to the determination of L-lactate in serum samples without dilution. This disposable biosensor combined with portable meter is promising for rapid point-of-care lactate determinations.

关 键 词：生物传感器 乳酸氧化酶 媒介体 铂黑纳米粒子 化学修饰电极 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]