黄芩苷对脑出血大鼠脑内氨基酸递质含量的影响  

Effect of Baicalin on the Content of Major Amino Acid Transmitters in the Brain of Rat with Experimental Intracerebral Hemorrhage

作　　者：周乾坤[1] 余嗣明[1] 刘平[1] 隗义双[1] 马树梅[1] 祝庆军[1] 赵艳梅[1] 

机构地区：[1]安徽大学生命科学学院,安徽大学中药研究与开发重点实验室,安徽合肥230039

出　　处：《中国中医药信息杂志》2009年第4期35-37,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽省自然科学基金(2004kj025);安徽大学创新团队计划(02203109)

摘　　要：目的观察黄芩苷对实验性脑出血大鼠脑内谷氨酸(Glu)、天冬氨酸(Asp)和γ-氨基丁酸(GABA)3种氨基酸含量的影响。方法采用胶原酶+肝素钠法诱导脑出血模型,分为假手术组、模型组及黄芩苷高、中、低剂量组,分别给予生理盐水、不同剂量黄芩苷灌胃,3d后取出各组大鼠大脑,制备脑匀浆,用高效液相色谱仪检测3种氨基酸含量。结果与假手术组比较,模型组和黄芩苷低剂量组Asp含量明显升高(P<0.05),不同剂量黄芩苷组与模型组比较,Asp有所下降(P>0.05);与假手术组比较,模型组和黄芩苷低、中剂量组Glu含量明显升高(P<0.05),与模型组比较,黄芩苷中、高剂量组Glu含量明显下降(P<0.05);与假手术组比较,模型组、黄芩苷低、中、高剂量组GABA含量均明显升高(P<0.05),与模型组比较,黄芩苷中、高剂量组GABA含量明显升高(P<0.05)。结论黄芩苷可能通过降低兴奋性氨基酸的含量,提高抑制性氨基酸的含量,来调节兴奋性/抑制性氨基酸的平衡,起到保护脑出血大鼠脑内神经细胞的作用。Objective To study the effect of baicalin on the content of glutamate acid （Glu）, aspartic acid （Asp）, y-aminobutyric acid （GABA） in the brain of rat with experimental intracerebral hemorrhage. Methods The intracerebral hemorrhage rat model was induced by collagen enzyme ＋ heparin sodium. The rats were randomly divided into sham group, model group and high, middle, low dose group of baicalin. Rats were treated with the physiological saline and different concentration of baicalin respectively. The brain of each group was taken out three days later and the brain homogenate was prepared. The content of Glu, Asp and GABA was measured with the high performance liquid chromatography （HPLC）. Results Compared with the sham group, the levels of Asp were obviously higher （P〈0.05） in the model group and low dose group of baicalin. Compared with the model group, Asp was decreased with no statistics significance （P〉0.05） in each dose group of baicalin. Compared with the sham group, the contents of G1u were increased obviously （P〈0.05） in the model group and low, middle dose group of baicalin. Compared with model group, the contents of Glu were decreased obviously （P〈0.05） in high and middle dose group. Compared with the sham group, the contents of GABA were obviously increased {P 〈0.05） in the model group and high, middle, low dose group of baicalin. Compared with the model group, the contents of GABA were significantly increased （P〈0.05） in high and middle dose group of baicalin. Conclusions Baicalin can regulate the balance between excitatory and inhibitory amino acid to protecting rat nerve ceU in the brain of intracerebrai hemorrhage by increasing the content of inhibitory amino acid and decreasing the content of excitatory amino acid.

关 键 词：黄芩苷 脑出血 谷氨酸 天冬氨酸 Γ-氨基丁酸 高效液相色谱法 大鼠 

分 类 号：R285.5[医药卫生—中药学]