大孔吸附树脂分离纯化长春花中长春碱和长春新碱  被引量:7

Isolation and Purification of Vinblastine and Vincristine from Gatharanthus Roseus by Macroporous Adsorption Resin

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作  者:潘岳峰[1] 王慧敏[2] 张玥[1] 韩静[1] 张立军[3] 殷莉梅[1] 杨静[1] 

机构地区:[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]沈阳兴齐制药有限公司,辽宁沈阳110027 [3]吉林敖东延边药业股份有限公司检测中心,吉林延边133700

出  处:《中国中医药信息杂志》2009年第4期51-53,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘  要:目的优选分离纯化长春花中长春碱和长春新碱的最佳工艺参数。方法采用不同型号大孔吸附树脂对长春花中长春碱和长春新碱分离纯化,用不同浓度乙醇水溶液以不同速度洗脱,配合硅胶柱层析,以HPLC跟踪检测。结果AB-8型树脂分离效果佳,90%乙醇水溶液以1.5BV/h的速度洗脱,总比吸附量达到74.5mg/g,经硅胶柱层析后长春新碱纯度达97.26%,长春碱纯度达94.18%。结论该工艺简便合理,重复性良好,能得到高纯度的长春碱和长春新碱。Objective To optimize the technological parameters of separation and purification of vinblastine and vincristine from Gatharanthus Roseus. Methods Different types of macroporous adsorption resin were used to separate and purify vinblastine and vincristine from Gatharanthus Roseus, eluting with different concentration of alcohol aqueous and velocities, combined with silica gel column chromatography, the process was monitored by HPLC. Results AB-8 type resin showed better comprehensive adsorption property, 90% alcohol aqueous and 1.5 BV/h velocities were used to elute. Ratio adsorption quantity was achieved to 74.5 mg/g. Through silica gel column chromatography, the purity of vincristine was achieved to 97.26% and the purity of vinblastine was achieved to 94.18%. Conclusions The process is simple and reasonable with good reproducibility. It is effective to enrich highly purified vinblastine and vincristine.

关 键 词:大孔吸附树脂 长春花 长春新碱 长春碱 

分 类 号:R283.5[医药卫生—中药学]

 

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