恩诺沙星残留对土壤细菌种群基因多样性的影响  

Effects of enrofloxacin on DNA sequence diversity of soil cultural bacterial community

作　　者：马驿[1,2] 陈杖榴[1] 

机构地区：[1]华南农业大学广东省兽药研制与安全评价重点实验室,广州510642 [2]广东海洋大学动物医学系,广东湛江524088

出　　处：《应用生态学报》2009年第4期949-954,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金重点项目(30130140)资助

摘　　要：为了解恩诺沙星在环境中残留对土壤微生物的影响,应用扩增核糖体DNA限制性分析(ARDRA)对土壤细菌16SrDNA基因多样性进行了研究,并结合肠杆菌基因间的重复共有序列(ERIC-PCR)指纹图谱分析了恩诺沙星对土壤细菌种群基因多样性的影响.结果表明:恩诺沙星作用于土壤后第35天,添加药物组的细菌总数均低于对照,且药物浓度越高,细菌数量越少;ARDRA分析将分离的土壤细菌分成了不同的操作分类单元(OTU),各组的OTUs类型数分别为:Ⅰ组15个、Ⅱ组13个、Ⅲ组10个、Ⅳ组8个、Ⅴ组6个、Ⅵ组6个;对各组优势OTU进行了ERIC-PCR基因指纹图谱分析,Ⅰ～Ⅵ组的Shannon-Wiener指数分别为2.78、2.14、1.78、1.11、0.69和0.31,对照组的Margalef指数、Simpson指数和Pielou指数均明显高于添加药物组,且各多样性指数随药物浓度的增加而减少.In order to understand the effects of remained enrofloxacin （ENR） in environment on the diversity of soil microbial communities, amplified ribosomal DNA restriction analysis （ARDRA） approach and genomic fingerprinting technique enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction （ERIC-PCR） were used to analyze the molecular diversity of 16S rDNA from soil bacteria after ENR addition. The results showed that after the ENR addition for 35 days, the total count of soil bacteria was less than that of CK, and decreased with increasing ENR concentration. The ARDRA divided the separated soil bacteria into different operational taxonomic units （OTU） groups, and the count of group Ⅰ to group Ⅵ was 15, 13, 10, 8, 6, and 6, respectively. Genomic fingerprinting analysis indicated that the Shannon-Wiener index of group Ⅰ to group Ⅵ was 2.78, 2. 14, 1.78, 1.11, 0. 69 and 0. 31, respectively, and the Margalef index, Simpson index, and Pielou index of soil microbial community in CK were higher than that in the soils in which ENR was added.

关 键 词：恩诺沙星 土壤微生物多样性ARDRA ERIC-PCR 

分 类 号：S154.3[农业科学—土壤学]