补阳还五汤对异丙肾上腺素诱导大鼠心肌缺血的保护作用  

Protective Effect of Buyanghuanwu Decoction on Myocardial Ischemia Induced by Isoproterenol in Rats

作　　者：刘瑜[1] 林蓉[1] 张慧[1] 张继业[1] 纪巧丽[1] 杨银京[2] 

机构地区：[1]西安交通大学医学院药理学系,陕西西安710061 [2]西安交通大学医学院药学系,陕西西安710061

出　　处：《中药材》2009年第3期380-383,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(90709018);陕西省中医药管理局重点项目(2005021)

摘　　要：目的:研究补阳还五汤对异丙肾上腺素诱导大鼠心肌缺血的保护作用。方法:大鼠灌胃给予补阳还五汤,腹腔注射异丙肾上腺素制备心肌缺血模型,流式细胞技术检测外周血CD40的表达,免疫组织化学法观察心肌组织CD40L的表达,并测定血清LDH、CK、AST的活性。结果:心肌缺血模型组大鼠外周血CD40、心肌组织CD40L的表达及血清LDH、CK、AST的活性明显高于正常对照组(P<0.01),而补阳还五汤高、中剂量组大鼠外周血CD40、心肌组织CD40L的表达及血清LDH、CK、AST的活性较模型组显著降低(P<0.01)。结论:补阳还五汤对异丙肾上腺素诱导的大鼠心肌缺血具有保护作用,其作用可能与阻断CD40-CD40L炎症信号途径及降低血清LDH、CK与AST的活性有关。Objective：To study the protective effect of Buyanghuanwu Decoction on myocardial ischemia induced by isoproterenol in rats. Methods： Buyanghuanwu Decoction was given in different dose and the rat model of myocardial ischemia was established by peritoneal injection of isoproterenol. The expression of CD40 in whole blood was detected by flow cytometry, and the expression of CIMOL in myocardial tissues was detected by immunohistochemistry. The activities of lactate dehydrogenase（ LDH）, creatine kinase（CK） and aspartate aminotransferase （AST）in blood serum were detected by biochemistry detector. Results：Compared with the model group, Buy- anghuanwu Decoction in high and middle dose significantly inhibited the expression of CD40 in blood serum and CD40L in myocardial tissues（P 〈 0. 01 ）, and obviously decreased the activities of LDH, CK and AST in blood serum（ P 〈 0. 01 ）. Conclusion： Buyanghuanwu Decoction has a protective effect on myocardial ischemia induced by isoproterenol in rats, and it may be relevant to the decrease of the expression of CIM0-CIMOL and the activities of myocardial enzymes.

关 键 词：补阳还五汤 异丙肾上腺素 心肌缺血 CIMO CIMOL 

分 类 号：R285.5[医药卫生—中药学]