中药复方有效部位的体外抗流感病毒作用  

Antiviral Effects of an Effective Section of a Prescription of Traditional Chinese Medicine on Influenza Virus A in vitro

作　　者：黄升海[1,2] 冯晓亮[1] 张玲玲[2] 许淑萍[2] 吴成义[2] 魏伟[2] 

机构地区：[1]安徽医科大学基础医学院微生物学教研室 [2]安徽医科大学临床药理研究所,安徽合肥230032

出　　处：《中药材》2009年第3期391-394,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究中药复方有效部位(TCM-ES)的体外抗流感病毒FM1株(IFV-FM1)的作用。方法:采用微量细胞病变抑制法、中性红染色法、空斑抑制法,从TCM-ES的最大无毒浓度开始,按Reed-Muench法计算药物对FM1感染狗肾传代细胞(MDCK)的半数有效浓度(EC50),最小有效浓度(MTC),治疗指数(TI)。进行空斑计数(PFU),计算其半数抑制浓度(IC50)。结果:微量细胞病变抑制法表明,TCM-ES抗FM1的EC50为(300±18.3)mg/L,MTC为(75±6.8)mg/L,TI为(7.1±0.7);而利巴韦林的3项指标分别为(52.3±10.1)mg/L、(25±4.1)mg/L和(20.8±5.1)。中性红染色法显示,两药物的IC50分别为(285.0±19.2)mg/L和(45.3±4.9)mg/L,TI分别为(7.2±0.6)和(21.2±3.1)。两种方法测定的TCM-ES和利巴韦林的TI值无统计学差异(P>0.05)。通过PFU计数,TCM-ES的半数抑制浓度(IC50)为300mg/L,利巴韦林的IC50为50mg/L,与前述结果亦无统计学差异(P>0.05)。结论:TCM-ES体外具有一定的抗流感病毒作用,可作用于细胞内病毒,但其效果略差于利巴韦林,具有进一步研究和开发的价值。Objective：To study the inhibitive effects of an effective section of a prescription of traditional Chinese medicine（ TCM- ES） on influenza virus A FM1 strain in vitro. Methods：The experiments were performed by microcytopathic-inhibiting-assay, Neutral Red stain and inhibiting plaque-forming units （PFU） test on MDCK cell strain. By means of observing the eytopathic effects （CPE）, measuring the absorbanee [ D（ A ）] and counting the PFU, according to Reed-Muench assay, the TCM-ES＇s effective dosage of 50 per-centage （ECso） and treatment index （TI） to FM1 were calculated. The inhibiting dose of 50 percentage of PFU （ICso） was also fig- ured up. Results：By CPE assay, TCM-ES＇S ECso, MTC and TI to 100TCIDs0 FM1 strain infection were （300 ± 18. 3 ）mg/L, （75 ± 6. 8 ） mg/L and （7. 1 ± 0. 7 ）, respectively; Whereas, ribavirin＇s ECso, MTC and TI was （ 52.3 ± 10. 1 ） rag/L, （25 ± 4. 1 ） mg/L and （20. 8 ± 5. 1 ）, respectively. By Neutral Red stain assay, TCM-ES＇ s ICso and TI was （ 285.0 ± 19. 2 ） mg/L and （7. 2 ± 0. 6 ）, respectively; Whereas ribavirin＇s IC50 and TI was（45.3 ± 4. 9 ）mg/L and （21.2 ± 3.1 ）, respectively. By reducing PFU assay,The ICso of TCM-ES and ribavirin was 300 mg/L and 50 mg/L, respectively. All the results above were almost consistent with each other（P 〉 0. 05）. Conelusions：TCM-ES assumes antiviral action on IFV-FMI strain in a certain degree in vitro and can rebel intracellular virus. But it is worse than the positive control medicine of ribavirin and is worthy of further study.

关 键 词：流行性感冒病毒 中药 有效部位 抗病毒作用 细胞病变效应 

分 类 号：R285.5[医药卫生—中药学]