Proteomic Study for Responses to Cadmium Stress in Rice Seedlings  

作　　者：GE Cai-lin WANG Ze-gang WAN Ding-zhen DING Yan WANG Yu-long SHANG Qi LUO Shi-shi 

机构地区：[1]Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China [2]College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China

出　　处：《Rice science》2009年第1期33-44,共12页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30300026)

摘　　要：A proteomic approach including two-dimensional electrophoresis and mass spectrometric （MALDI-TOF MS） analyses was used to investigate the responses to cadmium （Cd） stress in seedlings of rice （Oryza sativa L.） varieties Shanyou 63 and Aizaizhan. Cd stress significantly inhibited root and shoot growth, and affected the global proteome in rice roots and leaves, which induced or upregulated the expression of corresponding proteins in rice roots and leaves when rice seedlings were exposed to 0.1 or 1.0 mmol/L Cd. The Cd-induced proteins are involved in chelation and compartmentation of Cd, elimination of active oxygen free radicals, detoxification of toxic substances, degradation of denatured proteins or inactivated enzymes, regulation of physiologic metabolism and induction of pathogenesis-related proteins. Comparing the Cd-induced proteins between the two vadeties, the β-glucosidase and pathogenesis-related protein family 10 proteins were more drastically induced by Cd stress in roots and leaves of Aizaizhan, and the UDP-glucose protein transglucosylase and translational elongation factor Tu were induced by 0.1 mmol/L Cd stress in roots of Shanyou 63. This may be one of the important mechanisms for higher tolerance to Cd stress in Shanyou 63 than in Aizaizhan.A proteomic approach including two-dimensional electrophoresis and mass spectrometric （MALDI-TOF MS） analyses was used to investigate the responses to cadmium （Cd） stress in seedlings of rice （Oryza sativa L.） varieties Shanyou 63 and Aizaizhan. Cd stress significantly inhibited root and shoot growth, and affected the global proteome in rice roots and leaves, which induced or upregulated the expression of corresponding proteins in rice roots and leaves when rice seedlings were exposed to 0.1 or 1.0 mmol/L Cd. The Cd-induced proteins are involved in chelation and compartmentation of Cd, elimination of active oxygen free radicals, detoxification of toxic substances, degradation of denatured proteins or inactivated enzymes, regulation of physiologic metabolism and induction of pathogenesis-related proteins. Comparing the Cd-induced proteins between the two vadeties, the β-glucosidase and pathogenesis-related protein family 10 proteins were more drastically induced by Cd stress in roots and leaves of Aizaizhan, and the UDP-glucose protein transglucosylase and translational elongation factor Tu were induced by 0.1 mmol/L Cd stress in roots of Shanyou 63. This may be one of the important mechanisms for higher tolerance to Cd stress in Shanyou 63 than in Aizaizhan.

关 键 词：RICE CADMIUM PROTEOME stress response two-dimensional electrophoresis peptide mass fingerprints 

分 类 号：S511.01[农业科学—作物学] Q51[生物学—生物化学]