银杏叶提取物EGb761对局灶性脑缺血再灌注大鼠XIAP和Smac表达的影响  

Effects of Cinkgo biloba extract EGb/61 on the expressions of XIAP and Smac following focal cerelral ischemia/reperfusion in rats

作　　者：曹立梅[1] 毕桂南[2] 罗传铭[3] 陈旭[1] 

机构地区：[1]上海市第八人民医院神经内科 [2]广西医科大学第一附属医院神经内科,530021 [3]东莞市虎门医院,523900

出　　处：《国际脑血管病杂志》2009年第3期161-165,共5页International Journal of Cerebrovascular Diseases

基　　金：广西自然科学基金资助（桂科自0542091）

摘　　要：目的探讨银杏叶提取物EGb761对局灶性脑缺血大鼠脑组织X－连锁凋亡蛋白抑制剂（X—linked inhibitor of apoptosis protein，XIAP）和Smac蛋白表达的影响。方法40只雄性Wistar大鼠随机分为假手术组、脑缺血再灌注组、EGb761小剂量组和EGb761大剂量组，每组10只。制作大鼠大脑中动脉闭塞1．5h再灌注24h模型。EGb761小剂量组和EGb761大剂量组于模型制作前1h分别腹腔注射EGb76150mg／kg和100mg／kg。大鼠脑组织XIAP和Smac表达用免疫组化方法检测。结果EGb761小剂量组和EGb761大剂量组脑组织XIAP表达分别为18．33±4．01和26．7±3．27，屁著高于脑缺血再灌注组的12．13±3．44（P均〈0．01），且EGb761大剂量组高于EGb761小剂量组（P〈0．01）；EGb761小剂量组和EGb761大剂量组脑组织Smac表达分别为21．33±3．15和11．33±2．10，显著低于脑缺血再灌注组的28．93±4．96（P均〈0．05），EGb761大剂量组显著低于EGb761小剂量组（P〈0．01）。结论脑缺血再灌注可诱导XIAP和Smac表达，EGb761干预在上调XIAP表达的同时能抑制Smac蛋白表达，提高XIAP／Smac比值，可能是EGb761干预的保护机制之一。Objeclive To investigate the effects of Ginkgo biloba extract EGb761 on the expressions of XIAP and Smac following focal cerebral ischemia in rats. Methods A total of 40 male Wistar rats were randomized into 4 groups：sham-operation group, cerebral ischemia/reperfusion group, low-dose EGb761 group, and high-dose EGb761 group （n = 10 in each group）. A rat model of middle cerebral artery occlusion （MCAO） for 1.5 hours and reperfusion for 24 hours was built. EGb761 50 mg/kg and 100 mg/kg were injected intraperitoneally at one hour before the model building in the low-dose EGb761 and high-dose EGb761 groups. The expressions of XIAP and Smac in brain tissue were detected by immunohistochemistry method. Results The expressions of XIAP were 18. 33 ± 4. 01 and 26. 7 ± 3.27 respectively in the lowdose EGb761 and high-dose EGb761 groups, and they were significantly higher than 12.13 ±3.44 in the cerebral ischemia/reperfusion group （all P 〈 0.01 ）, and the high-dose EGb761 group was higher than the low-dose EGb761 group （P 〈0.01）. The expressions of Smac in brain tissue were 21.33± 3.15 and 11.33 ± 2. 10 respectively in the low-dose EGb761 and high-dose ECb761 groups, and they were significantly lower than 28.93 ±4.96 in the cerebral ischemia/reperfusion group （all P 〈0.05）. The high-dose EGb761 group was significantly lower than the low-dose EGb761 group （P 〈 0.05）. Conclusions Cerebral ischemia/reperfusion could induce the expressions of XIAP and Smac. EGb761 intervention could inhibit the expressions of Smac while upregulating the expression of XIAP, and increase the XIAP/Smac ratio. This may be one of the protective mechanisms of EGb761 intervention.

关 键 词：银杏 XIAP SMAC 脑缺血 

分 类 号：R285.5[医药卫生—中药学]