高糖对培养大鼠施万细胞生长、凋亡的影响及其机制探讨  

Effects and mechanism of high-glucose on growth and apoptosis of Schwann cells in vitro

作　　者：乐嫣[1] 朱浩[1] 王文进[1] 丁文龙[1] 

机构地区：[1]上海交通大学医学院解剖学教研室上海交通大学医学科学院,上海200025

出　　处：《解剖学报》2009年第2期246-249,共4页Acta Anatomica Sinica

基　　金：上海市重点学科建设资助项目(S30201)

摘　　要：目的研究高糖对大鼠施万细胞（SCs）生长及凋亡的影响,并探讨其可能机制。方法将培养的SCs分别用含不同浓度葡萄糖的培养液孵育72h,四甲基偶氮唑盐（MTT）检测细胞活力,原位缺口末端标记法（TUNEL）检测细胞凋亡,Western blotting检测Bcl-2及Bax的表达。结果高浓度葡萄糖（60mmol/L、90mmol/L）组SCs活力降低,细胞凋亡率增加,抗凋亡蛋白Bcl-2表达下降及凋亡蛋白Bax表达升高。结论高糖抑制SCs生长,诱导细胞凋亡,可能与Bcl-2表达下降及Bax表达升高有关。Objective To study the effects and possible mechanism of high-glucose on rat Schwann cells growth and apoptosis in vitro. Methods Schwann cells were cultured with different concentrations of glucose for 72 hours. MTT assays were used to detect the activities of SCs. Apoptosis was observed by TUNEL assay. The expressions of Bcl-2 and Bax protein were evaluated by Western blotting assay. Results In high-glucose （60mmol/L,90mmol/L） group,the activity of SCs was decreased and the rate of apoptosis was increased. The expression level of Bcl-2 in SCs was decreased while Bax expression level was increased in high glucose culture. Conclusion High glucose inhibits the growth of SCs and increases the cell apoptosis,which is possibly caused by decreasing the expression of Bcl-2 and up-regulating the expression of Bax.

关 键 词：高糖 施万细胞 Bcl-2 BAX TUNEL 免疫印迹法 大鼠 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]