兔下颌骨牵张成骨中HIF-1α和c-fos的表达及其相关性  

Expressions of HIF-1α,c-fos and their relationships in mandibular distraction osteogenesis in rabbits

作　　者：赵翚[1] 谢志坚[2] 何剑锋[2] 葛巍立[2] 林坚[1] 胡济安[2] 

机构地区：[1]温州市第三人民医院口腔颌面外科,浙江温州325000 [2]浙江大学附属口腔医院颌面外科,杭州310006

出　　处：《解剖学报》2009年第2期299-302,共4页Acta Anatomica Sinica

基　　金：浙江省科技厅资助项目(2007C30041,2007C-23016);温州市科技局资助项目(Y20060060);浙江省卫生高层次创新人才培养工程资助项目(2008A)

摘　　要：目的探讨兔下颌骨牵张成骨中HIF-1α和c-fos的表达及其相关性。方法建立25只成年健康家兔下颌骨牵张成骨模型,采用免疫组织化学方法,分别在牵张中期、牵张末期、固定期第1、3、5周,检测牵张区新骨组织中HIF-1α和c-fos的分布及表达变化。结果HIF-1α在牵张末期和固定第1周表达为强阳性,c-fos在牵张中、末期和固定第1周表达为强阳性,两者均明显强于固定第3、5周。且均主要表达于成纤维细胞、成骨细胞和新埋入骨基质中的骨细胞中。结论在下颌骨缺损牵张成骨中HIF-1α和c-fos在细胞中的分布及在时间点的表达具有显著的相关性,对骨及血管的生成可能起着重要的生物学作用。Objective To investigate the expressions of HIF-1α,c-fos and their relationships in mandibular distraction osteogenesis in rabbits. Methods To establish an animal model of mandibular distraction osteogenesis with 25 healthy adult rabbits.The expressions of HIF-1α and c-fos were detected by ultrasensitive SP immunohistochemical methods. Results Immunohistochemical studies showed that the HIF-1α was expressed strongly in distraction zone at the 8th days after distraction. One week after consolidation,the c-fos was expressed strongly in the distraction zone at the 4 th day,8th days after distraction,and 1 week after consolidation.Both apparently expressed apparently higher than those in the distraction zone of at the third and fifth week after consolidation. HIF-1α and c-fos were mainly expressed in fibroblasts,osteoblasts and osteocytes. Conclusion In mandibular distraction osteogenesis of rabbits,there is a marked relationship between HIF-1α and c-fos expressions in the site and the time. Thus ,they may play a critical roles in angiogenesis and osteogenesis.

关 键 词：HIF-1Α C-FOS 下颌骨 牵张成骨 免疫组织化学 兔 

分 类 号：R332[医药卫生—人体生理学]