低剂量棉酚与甾体激素联合用药抗男性生育协同作用  

Synergistic effect of combined regimen of low-dose gossypol with steroid hormones on male antifertility

作　　者：常青[1] 邵金辉[1] 许增禄[1] 张成侠[1] 钱晓菁[1] 

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院人体解剖学与组织胚胎学系

出　　处：《解剖学报》2009年第2期312-316,共5页Acta Anatomica Sinica

摘　　要：目的探讨低剂量棉酚和甾体激素联合用药发挥抗男性生育的药物协同作用。方法采用大鼠喂服棉酚与甾体激素联合用药方式[棉酚12.5mg/（kg·d）＋去氧孕烯125μg/（kg·d）＋炔雌醇25μg/（kg·d）＋十一酸睾丸酮100mg/（kg·d）],与单独喂服相同剂量的棉酚或激素的大鼠及喂服载体溶剂的大鼠相对照,应用形态学观察、半定量检测和体视学睾丸组织结构定量研究的方法,探讨低剂量棉酚和甾体激素抗生育的药物协同作用。结果在睾丸重量、附睾重量、睾丸精子产量和睾丸组织结构定量方面,低剂量棉酚和甾体激素无药物协同作用;在降低附睾精子计数和精子活动度方面,联合用药组的效果强于其他3组,低剂量棉酚和甾体激素具有药物协同作用。结论与单用低剂量棉酚和甾体激素比较,两者联合用药可以更快地减少附睾精子的数量和活动度,从而快速达到避孕效果。Objective To study the synergistic effect on male antifertility of a combined regimen of low-does gossypol with steroid hormones. Methods Adult male rats were divided randomly into four groups：group GH,rats were fed orally with GA [12.5mg/（kg·d）] and DSG [0.125mg/（kg·d）]/E [0.025mg/（kg·d）]/TU[100mg/L（kg·d）]; group G,a single does of GA [12.5mg/（kg·d）] was given; group H,DSG [0.125mg/（kg·d）]/E[0.025mg/（kg·d）]/TU[100mg/（kg·d）] were administered; group C,rats only received vehicle （1% methyl cellulose）. The testis and epididymis were removed at 4,6 and 8 week for counting sperm number,sperm motility and stereological changes of testis tissue. Results Low-dose gossypol with steroid hormones did not have a synergistic effect on reducing testicular weight,sperm production or stereological parameters. However,they did have a synergistic effect on reducing epididymal sperm number and sperm motility. Conclusion A combined regimen of low-dose gossypol with steroid hormones can cause a more rapidly reduction of the number of epididymal sperm and sperm motility than only using gossypol or steroid hormones. Thus the combined regimen has a faster and better anti-fertility effect on male rats.

关 键 词：棉酚 甾体激素 抗男性生育 协同作用 大鼠 

分 类 号：R979.2[医药卫生—药品]