视神经切断联合高眼压增高大鼠视网膜内P75^(NTR)和Sortilin的蛋白表达水平  

Optic nerve transaction combined with high intraocular pressure increased the protein expression levels of P75^(NTR) and sortilin in retina of rats

作　　者：鹿宁宁[1] 王宁利[1] 魏勇[1] 丁静文[1] 韩松[2] 李俊发[2] 

机构地区：[1]首都医科大学北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,北京100730 [2]首都医科大学神经生物学系北京神经科学研究所,北京100069

出　　处：《基础医学与临床》2009年第4期347-351,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30571991)

摘　　要：目的观察视神经切断联合高眼压状态下,视网膜内P75NTR和Sortilin蛋白表达水平的变化。方法60只健康雄性Wistar大鼠,随机分为对照(control,n=6)、假手术(sham,n=6)、单纯视神经切断(NT,n=24)和视神经切断联合高眼压(NP,n=24)4组。NT和NP模型组,进一步分为术后第1、3、7和14天组(均n=6)。用Western blot法,检测各组大鼠视网膜内P75NTR和Sortilin蛋白表达。结果NP组大鼠视网膜内,P75NTR及Sortilin蛋白表达量在术后第3、7和14天明显高于对照组、假手术组及单纯视神经切断组(P<0.05)。结论高眼压可使大鼠视网膜内RGC本身合成的Sortilin和P75NTR蛋白量增高,60/50 ku的P75NTR,以及90 ku成熟Sortilin蛋白可能参与了高眼压所致大鼠视网膜神经节细胞的损伤。Objective To observe the changes in sortilin and P75^NTR protein expressions in rat retina following optic nerve transaction combined with high intraocular pressure. Methods Sixty healthy male Wistar rats, were randomly divided into 4 groups ： the control （ control, n = 6）, sham （ n = 6）, optic nerve transaction （ NT, n = 24）, and NT combined with high intraocular pressure （ NP, n = 24） groups. In addition, the NT and NP groups were subdivided into 1, 3, 7 and 14 days＇ group （n = 6 for each group）. The P75^NTR and sortilin protein expression was determined by Western blot. Results The protein expressions of P75^NTR, and sortilin increased significantly （ P 〈 0. 05 ） in retina of rats from NP group at day 3,7 and 14 as compared with that of control, sham and NT groups. Conclusion The increased P75 ^NTR and sortilin protein expressions in retina of rats following high intraoeular pressure came from retinal RGC themselves. These results also suggested that the enhanced sortilin （ 90 ku ） and P75^NTR （ 6 0 / 50 ku ） protein expressions might be involved in the high ocular pressure-induced retinal RGC injuries of rats.

关 键 词：视神经切断 高眼压 青光眼 P75^NTR SORTILIN 

分 类 号：R779.6[医药卫生—眼科]