微米级磨损微粒刺激溶骨性细胞因子表达  

Otinulation of osteolytic cytokine expression by micrometer-diameter wear particles

作　　者：陈明[1,2] 董启榕[1] 雷宇[3] 

机构地区：[1]苏州大学第二附属医院骨科,江苏苏州215004 [2]南昌大学第一附属医院骨科,江西南昌330006 [3]华东交通大学职业技术学院信息系,江西南昌330013

出　　处：《基础医学与临床》2009年第4期366-370,共5页Basic and Clinical Medicine

基　　金：卫生部科学研究基金(WKJ2004-2-009)

摘　　要：目的比较钛合金和超高分子质量聚乙烯两种微米级磨损微粒对溶骨性细胞因子表达的影响。方法每2天1次于大鼠背部皮下注射过滤后的空气3 mL,共6次。1周后,囊腔内分别注射微粒悬液钛合金(A组),超高分子质量聚乙烯(B组)和生理盐水(C组)。14 d后取囊腔组织称重,石蜡包埋、HE染色,光镜下观察,全自动生化分析仪测定血清AKP活性,免疫组化法检测IL-6及TNF-α的表达,实时定量PCR法检测细胞外基质金属蛋白酶诱导因子(EMMPRIN)的mRNA含量。结果囊腔组织类似于无菌性松动假体周围界膜组织。B组重量高于C组(P<0.05)。光镜下,A、B组可见大量吞噬细胞。A、B组血清AKP活性、IL-6的表达和EMMPRIN的mRNA含量均高于C组(P<0.05)。结论钛合金和超高分子质量聚乙烯微粒均能刺激溶骨性细胞因子产生及活性升高,形成人工关节无菌性松动。Objective To evaluate the impact osteolytic cytokines of expression induced by micrometer-diameter wear particles （Ti-6Al-4V and UHMWPE）. Methods Filtration air was subcutaneously injected into rats＇back 6 times （3 mL q · d）. After a week, wear particles suspension （ group A ： Ti-6Al-4V, group B ： UHMWPE ） or physiological saline（ group C） was injected into air pouch tissues. After 14 days, pouch tissues were obtained from killed rats, and were weighted, wax embedded and stained with hematoxylin and eosin, observed under microscope. AKP of serium with Automated Biochemical Analyzer, IL-6 and TNF-α expression with immunohistochemical method, and mRNA expression of extracellular matrix metalloproteinase inducer （EMMPRIN） with real-time Polymerase Chain Reaction method were detected. Results Air pouch tissues were similar to limiting membrane of periprothesis tissue in the cases of aseptic loosening. As to pouch tissue weight, there was a significant increase in group B than in group C （P 〈 0. 05 ）. Microscopy illustrated a large amount of phagocytes in group A and B. The activities of AKP, expression of IL-6 and mRNA expression of EMMPRIN in group A and group B were higher than in group C （P 〈 0. 05）. Conclusion Both two wear particles can modulate the production and expression of osteolytic cytokines leading to aseptic loosening.

关 键 词：磨损微粒 动物模型 溶骨I生细胞因子 表达 

分 类 号：R318.08[医药卫生—生物医学工程]