低剂量γ射线辐照对血清抗氧化系统的兴奋效应  

Hormesis effect of serum antioxidative system after low dose human whole blood gamma ray irradiation

作　　者：夏爱军[1] 张献清[1] 穆士杰[1] 易静[1] 吴原茹[1] 

机构地区：[1]第四军医大学西京医院输血科,陕西西安710032

出　　处：《西北国防医学杂志》2009年第2期100-102,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:探讨低剂量γ射线辐照人全血对血清抗氧化酶活性的影响。方法:20份人全血,以剂量为1 Gy的γ射线进行辐照,吸收剂量率为17 Gy/min,分别于照射前及照射后1、2 h取样检测血清中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活力及还原性谷胱甘肽(GSH)、丙二醛(MDA)含量。结果:离体全血在剂量为1 Gyγ射线照射后1 h,可见血清中GSH含量升高,MDA含量降低及T-AOC、SOD、GR、GSH-Px、CAT活性升高,且均与照射前比较有显著差异(P<0.01)。经γ射线照射2 h后,除SOD活性与照射后1 h差异无显著性外,MDA含量进一步降低,GSH含量及T-AOC、GR、GSH-Px及CAT活性进一步升高,与照射后1 h比较有显著差异(P<0.01)。结论:低剂量γ射线辐照能明显提高血清抗氧化能力,即可诱导抗氧化系统的兴奋效应。Objective：To investigate the activities of serum anti - oxidative enzymes by human whole blood gamma ray irradiation . Methods： Twenty units of human whold blood were irradiated by γ- ray at a dose of 1Gy. The absorption dose rate was 17 Gy/min. The serum enzymatic activities of total antioxidative capacity（T-AOC）, superoxide dismutase （ SOD ）, glutathione peroxidase （ GSH - Px ）, catalase （ CAT）, glutathionereductase （ GR ）, contents of glutathione （ GSH ） and malondiadehyde （ MDA ） were deteted before and 1,2 h after irradiation respectively. Results： One hour after irradiation, the content of serum GSI-I was higher than that of pre - radiation （P 〈0.01 ）. The content of serum MDA was lower than that of pre -radiation （p 〈 0.01 ）. But the enzymatic activities of T - AOC, SOD, GSH - Px, GR and CAT were higher than that of pre - radiation （p 〈 0.01 ）. In two hours after radiation group, the content of serum GSH was higher than that of one hour after radiation group （P 〈 0. 01 ）, but content of serum MDA was lower（P 〈0.01 ）. All antioxidative enzyme activities were higher than that of one hour after radiation group except SOD （p 〈 0.01 ）. Conclusion： Gamma ray irradiation at low dose can obviously enhance the serum antioxidative abilities, that is to say, it results in the hormesis of antioxidation system.

关 键 词：Γ射线 抗氧化 照射 兴奋效应 

分 类 号：R144[医药卫生—公共卫生与预防医学]