卡马西平降解菌的筛选及降解特性研究  

Isolation of a Bacterial Strain Capable of Carbamazepine-degrading and Biodegradation Characteristics

作　　者：崔长征[1] 胡洪营[1] 于亚琦[1] 于茵[1] 

机构地区：[1]清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京100084

出　　处：《微生物学通报》2009年第4期557-562,共6页Microbiology China

基　　金：国家重点实验室专项基金课题(No.08Z02ESPCT);国家博士后科学基金项目(No.2008440367)

摘　　要：药品和个人护理品类污染物日益成为新兴污染物研究的重点,药品卡马西平因具有多种药效被广泛使用,在环境中频繁被检出,且浓度较高,不易去除,通常作为环境中药品和个人护理品污染状况的指示化合物。本研究从某制药厂的污水处理厂中分离到一株细菌HY-7,能以卡马西平为唯一碳源、氮源和能源生长,通过生理生化以及16SrDNA、gyrB基因序列分析鉴定并命名为Acinetobacter sp.HY-7。该菌株生长和降解卡马西平的最适条件为25°C和pH6.0,经HPLC分析10d内能将初始浓度为20mg/L的卡马西平降解48%。菌株HY-7还能以邻苯二酚、吲哚、萘、蒽等芳香族化合物为唯一碳源生长。Quite recently, among new emerging contaminants, pharmaceutical and personal care products （PPCPs） and their active metabolites are an emerging environmental issue, due to their presence in the aquatic environment and potential for impacts on wildlife and humans. Carbamazepine is one of the most frequently and at the relatively high concentration levels detected pharmaceuticals in surface water and even in drinking water. Moreover, this drug has displayed high degrading bacterium was isolated from activated sludge chronic ecotoxicity. A strain of carbamazepinetreating pharmaceutical wastewater in Suzhou, China. It was identified as Acinetobacter sp. HY-7, based on biochemical test, 16S rRNA and gyrB gene se- quence analysis. Strain HY-7 could grow in liquid mineral salt medium with carbamazepine as sole source of carbon, nitrogen and energy. HPLC analysis revealed the carbamazepine degradation percentage by HY-7 after 10 days was 48% at pH 6.0 and 25℃. Among carbamazepine and the similar structure compounds, indole, catechol, naphthalene, anthracene could also be utilized by strain HY-7 for growth, which exhibited a very broad substrate profile.

关 键 词：新兴污染物 药品和个人护理品 卡马西平 生物降解 不动杆菌 

分 类 号：Q93[生物学—微生物学]