地黄寡糖对谷氨酸诱导的海马神经元损伤及葡萄糖摄入的影响  

Effects of Rehmannia glutinosa oligosaccharides on damage and glucose uptake induced by glutamate in hippocampal neurons

作　　者：杨菁[1] 史佳琳[1] 白剑[1] 徐新力[1] 王洪新[2] 刘春娜[2] 

机构地区：[1]辽宁医学院细胞生物学及新药开发重点实验室,辽宁锦州121001 [2]辽宁医学院药理学教研室,辽宁锦州121001

出　　处：《中国药理学与毒理学杂志》2009年第2期99-103,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：辽宁省自然科学基金资助项目(20072202);辽宁省教育厅资助课题(20060528)~~

摘　　要：目的观察地黄寡糖(ROS)对谷氨酸(Glu)诱导海马神经元损伤及葡萄糖摄入的影响。方法将培养7d的SD大鼠海马细胞随机分为对照组、活性ROS用药组(4,20及100mg.L-1)、Glu损伤组(Glu0.1mmol.L-1)及Glu损伤前ROS预处理组(ROS+Glu)。采用倒置显微镜观察细胞形态学变化,噻唑蓝(MTT)法测定细胞存活率,比色法测定培养液中的乳酸脱氢酶(LDH)活性,[3H]葡萄糖掺入法测定神经元葡萄糖的摄取。结果单纯ROS4,20及100mg.L-1组与对照组间各指标差异没有显著性(P>0.05)。ROS4,20及100mg.L-1预处理可使Glu损伤的细胞存活率由70.4%分别上升为77.8%,85.2%,92.6%,同时也改善了神经细胞形态并减少LDH的漏出;[3H]葡萄糖掺入量也由Glu损伤组的(1547±120)分别下降为(1384±181),(1303±123),(1134±123)cpm。结论ROS对Glu引起的神经元损伤具有保护作用,这种保护作用可能与其抑制神经细胞对葡萄糖的过度摄入有关。AIM To investigate the effects of Rehmannia glutinosa oligosaccharides（ROS） on the damage and glucose uptake in hippocampal neurons induced by glutamate.METHODS The neurons isolated from hippocampus in new born SD rats were cultured for 7 d,which were randomly divided into 4 groups：normal cultures（control）,ROS group（ROS 4,20,100 mg·L^-1）;glutamate group（0.1 mmol·L^-1）,and ROS＋Glu group（pretreated with ROS）.The neurons＇ morphology was observed under inverted microscope;cell viability was assayed by MTT staining;LDH release was detected with chromatometry and glucose uptake was observed by [3H]glucose incorporation method.RESULTS There was no difference among ROS 4,20,100 mg·L^-1 and control group in all observational indexes.Pretreated with ROS 4,20 and 100 mg·L^-1,the cell viability was increased from Glu group＇s 70.4% to 77.8%,85.2% and 92.6%,respectively.The neurons＇ morphology was ameliorated and LDH release was decreased.Glucose uptake was decreased from Glu group＇s（1547±120） to（1384±181）,（1303±123） and（1134±123）cpm,respectively.CONCLUSION ROS can protect hippocampus neurons from the damage induced by glutamate,which may be related with inhibiting the neurons glucose uptake excessively.

关 键 词：地黄寡糖 谷氨酸 海马 神经元 葡萄糖摄取 

分 类 号：R285.5[医药卫生—中药学]