BN大鼠和豚鼠评价双黄连注射液的过敏反应  

Allergic response of Shuanghuanglian injection in BN rats and guinea pigs

作　　者：郭姗姗[1] 王意忠[2] 张毅[1] 李德凤[1] 宗桂珍[1] 高英杰[1] 时宇静[1] 苏丹[1] 崔晓兰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京大学航天临床医学院,北京100049

出　　处：《中国药理学与毒理学杂志》2009年第2期128-133,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金重大专项资助项目(90709042)~~

摘　　要：目的为摸索适合评价中药过敏反应的动物,采用BN大鼠和豚鼠评价双黄连注射液过敏的反应。方法BN大鼠和豚鼠分为ip或iv临床等倍剂量组(分别为360及300mg.kg-1)、iv临床2倍剂量组(分别为720及600mg.kg-1)。以双黄连注射液为致敏原,分别对2种动物进行致敏,隔日1次,共3次。同时设生理盐水对照组,观察各组过敏反应症状;采用ELISA法测量血清和组织中组胺含量,HE染色法光镜下观察肺组织和气管病理改变。结果双黄连注射液可以造成BN大鼠及豚鼠发生过敏反应,过敏反应发生率BN大鼠组明显高于豚鼠组,分别为62.86%和36.11%。双黄连注射液可使BN大鼠和豚鼠血清、肺和气管中组胺含量均明显升高,与各相应的对照组比较均具有显著性差异,同时,从数据上看,BN大鼠各组的组胺释放率均高于豚鼠各组。结论双黄连注射液可造成BN大鼠及豚鼠发生过敏反应。BN大鼠的敏感性可能高于豚鼠,但还需进一步研究。AIM To establish a suitable animal model to study allergic response induced by traditional Chinese materia medica.METHODS BN rats and guinea pigs were divided into intraperitoneal injection group（ip）,intravenous group（iv） given clinic equivalent dosage（360 and 300 mg·kg-1,respectively for 2 kinds animals） and iv group given 2 times clinic equivalent dosage（720 and 600 mg·kg-1,respectively for 2 kinds animals）.Sterile saline as control,animals were sensitized by Shuanghuanglian injection every other day,totally 3 times.The symptoms of allergic response were observed,the level of histamine in serum and tissues were determined by ELISA assay and pathological changes in lungs and trachea were observed by HE staining under light microscope.RESULTS Shuanghuanglian injection could induce allergic response in BN rats and guinea pigs.The total incidence of allergic response in BN rat groups was 62.86%,which was higher than that（36.11%） in guinea pig groups significantly.Compared with control groups,the level of histamine in serum,lungs and trachea tissues of BN rat groups and guinea pig groups increased significantly,but the release rate of histamine in BN rat groups was higher than that in guinea pig groups.In BN rat groups,the rate of pathological changes in lungs and trachea tissues was higher than that in guinea pig groups.CONCLUSIONShuanghuanglian injection can induce allergic response in BN rats and guinea pigs.BN rats might be more sensitive than guinea pigs in evaluating allergic response induced by injection of TCMM,but further studies should be done to draw the conclusion.

关 键 词：大鼠 近交BN 双黄连注射液 超敏反应 组胺 

分 类 号：R285[医药卫生—中药学]