羟基和超氧自由基的检测研究进展  

Advances in Determination of Hydroxyl and Superoxide Radicals

作　　者：张昊[1] 任发政[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院、教育部-北京市功能乳品实验室,北京100083

出　　处：《光谱学与光谱分析》2009年第4期1093-1099,共7页Spectroscopy and Spectral Analysis

基　　金：国家“十一五”科技支撑项目(2006BAD05A16)资助

摘　　要：活细胞在必需的新陈代谢过程中会产生自由基,越来越多的研究证据表明,这些自由基涉及到许多体内调控系统,然而一旦有过多的自由基生成便会氧化细胞脂膜、蛋白质、DNA和酶,进而对细胞造成致命性的损伤。此外,研究还表明许多疾病与自由基密切相关,例如,有研究报道海氏默症病人脑中生物分子的氧化损伤程度明显高于正常值,另外癌症可能也是DNA受到氧化损伤的结果。因此,测定自由基的方法就显得十分必需和重要。文章重点对羟基和超氧自由基检测技术的发展情况进行了讨论,涉及的自由基检测技术主要有分光光度法、荧光法、化学发光法和电子自旋共振技术,并评价了各种方法的优缺点。Metabolism is essential for the survival of cells with the production of free radicals. There is increasing evidence for the involvement of such species in a variety of normal in vivo regulatory systems. When an excess of free radicals is formed, they cause destructive and lethal cellular effects by oxidizing membrane lipids, cellular proteins, DNA and enzymes. In addition, it has been recognized that oxidative stress plays a significant role in a number of diseases. For example, many studies have shown increased oxidative damage to all the major classes of biomolecules in the brains of Alzheimer＇s patients. Furthermore, cancer is probably a consequence of oxidative DNA damage. Therefore, the determination methods of free radical are very important and necessary. The development of determination techniques of hydroxyl and superoxide radicals was reviewed with 62 references in the present paper, the techniques of determination mainly include spectrophotometry, fluorophotometry, chemiluminescence, and electron spin resonance, and their advantages and disadvantages were evaluated.

关 键 词：羟基自由基 超氧自由基 检测技术 评述 

分 类 号：O657.3[理学—分析化学]