固体进样石墨炉原子吸收光谱技术在测定大鼠脑铁含量中的应用  

Application of Solid Sampling Graphite Furnace Atomic Absorption Spectrophotometry to Mensuration of Brain Iron Content in Rats

作　　者：张楠[1] 生庆海[2] 石振华[1] 张志国[2] 段相林[1] 常彦忠[1] 

机构地区：[1]河北师范大学生命科学学院铁代谢分子生物学研究室,河北石家庄050016 [2]石家庄三鹿集团股份有限公司,河北石家庄050071

出　　处：《光谱学与光谱分析》2009年第4期1127-1130,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30570957);河北省自然科学基金项目(C2006000152,C2007000251)资助

摘　　要：石墨炉原子吸收光谱法是测定动物脑组织Fe含量的常用方法,然而由于传统的液体进样方法中,样品前处理阶段用硝酸消解周期长,成本高耗时长,限制了原子吸收光谱技术在测定动物组织中Fe含量的应用。文章以SD大鼠脑部海马、纹状体、皮层为实验材料,采用固体进样器进样,利用石墨炉原子吸收光谱技术对各组织中的Fe含量进行了测定,并与传统法方法——硝酸消解样品后,液体进样的石墨炉原子吸收光谱测定结果做了比较,结果表明这两种方法所测结果无显著性差异,但应用固体进样法,样品前处理阶段明显简单,显著节省了样品准备时间,降低了工作量,使测定时间明显缩短,成本明显降低,并且能够有效避免外界因素对测定结果的影响,为固体进样法的进一步应用奠定了基础。In the present study, the authors performed the solid sampling and detected the iron levels in cortex, hippocampus and striatum of rat brain by GFAAS. The authors＇ results showed that there are no remarkable difference between the data obtained by solid sampling graphite furnace atomic absorption and liquid sampling graphite furnace atomic absorption. Compared to liquid sampling graphite furnace atomic absorption, the sample pre-treatment stage was obviously simplified, the cost was reduced significantly, and the time was shortened significantly in the solid sampling GFAAS. This study will be beneficial to the mensuration of iron content in micro-tissue of animal by solid sampling GFASS.

关 键 词：石墨炉原子吸收光谱 固体进样 脑区 Fe含量 

分 类 号：O657.3[理学—分析化学]