板栗蛋白磷酸酶PP2A催化亚基基因的克隆与同源性分析  

Cloning and Homology Analysis of Chestnut Protein Phosphatase PP2A Catalytic Subunit Gene

作　　者：唐征[1] 杨凯[2] 冯永庆[1] 秦岭[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院农业应用新技术北京市重点实验室,北京102206

出　　处：《北京农学院学报》2009年第2期1-4,共4页Journal of Beijing University of Agriculture

基　　金：国家自然基金资助项目(30872039);北京自然基金资助项目(6082004)

摘　　要：利用RACE技术从板栗雄花序中扩增得到1 347 bp的板栗蛋白磷酸酶2A的催化亚基(protein phospha-tase PP2A catalytic subunit,PP2Ac)cDNA片段。序列分析表明该片段包含基因的5′非翻译区4 bp,3′非翻译区407 bp,开放阅读框为936 bp,编码311个氨基酸,预计分子量为35.6 KD,等电点为5.94。基因序列数据库(GenBanK)登录为FJ840479(基因)和ACO57639(蛋白)。与葡萄、拟南芥、水稻等其他物种PP2Ac氨基酸序列相似性约为92%。The gene was cloned from short catkins of Castanea mollissima Bl. by using rapid amplification of cDNA end （RACE） technology, and was designated as protein phosphatase PP2A catalytic subunit （PP2Ac）. The length cDNA of PP2Ac is 1 347 bp. It was found that this cDNA contained the complete coding sequence of PP2Ac of chestnut including 4 bp of 5t untranslated region, 407 bp of 3r untranslated region, and an open reading frame （ORF） of 936 bp. The ORF region encoded a peptide of 311 deduced amino acid residues. The deduced molecular weight of protein was 35.6 kD and its isoelectric point was 5.94. The gene was accepted by GenBank, the accession numbers were FJ840479 （gene） and ACO57639 （protein）. The sequence of this deduced polypeptide has a 92 % similarity to Vitis vinifera, Arabidopsis thaliana and Oryza sativa protein phosphatase PP2A catalytic subunit.

关 键 词：板栗 RACE 蛋白磷酸酶PP2A催化亚基 

分 类 号：S664.2[农业科学—果树学]