白术微管液相色谱含量测定方法及在种质资源调查中的应用  

Analysis of Atractylenolide in Artactylodis Macrocephala by Microbore Liquid Chromatography and its Application in Plant Resource Investigation

作　　者：寿旦[1] 戴诗文[1] 章建民[1] 俞忠明[1] 李洪玉[1] 

机构地区：[1]浙江省中医药研究院,浙江杭州310007

出　　处：《中华中医药学刊》2009年第1期61-62,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：国家十五攻关资助项目(2004BA721A21);浙江省科技计划资助项目(2008R20007)

摘　　要：目的:采用微管液相色谱法同时测定白术中的白术内酯Ⅲ、白术内酯Ⅰ和苍术酮,并应用于全国白术种质资源调查。方法:采用微管液相色谱仪,Microsil C18微管色谱柱(150mm×1.0mm),以甲醇-乙腈-水为流动相,梯度洗脱,流速:50μL/min,检测波长220nm,采用该方法,对全国20多个地区的白术种质样品进行了测定。结果:建立了同时分离白术中的白术内酯Ⅲ、Ⅰ和苍术酮的测定方法,测定了全国主产区白术种质的含量,初步得到了优质种源。结论:该方法准确、简便,在白术种质资源调查中起到了很好的作用。Objective： To establish a microbore Liquid Chromatography method for determining the content of atractylenolide Ⅲ, Ⅰ and atractylon in Artactylodis macrocephala,Which is furthermore applied in the investigation of plant resource. Methods ：In this microbore LC method, Microsil C18 column（ 150mm × 1.0mm）was used with the mobile phase containing methol-acetonitrile-water for gradient elution, the flow rate was 50μL/min, and the detection wavelength was set at 220nm. Results ： Under the above separation conditions, the contents of samples gathered from deferent area were determined in order to choose the high quality plant resource. Conclusion：The method is simple, reliable, and can be applied to value the plant resource

关 键 词：微管液相色谱法 白术内酯Ⅲ 白术内酯Ⅰ 苍术酮 

分 类 号：R284.1[医药卫生—中药学]