复方薤白胶囊对H_2O_2诱导的人脐静脉内皮细胞凋亡的研究  

Study of Compound Macrostem Onion Capsule on Apoptosis Induced by H_2O_2 in HUVEC

作　　者：张文曦[1] 奚肇庆[2] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《中华中医药学刊》2009年第1期93-96,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省中医药局重点科研项目(01074)

摘　　要：目的:研究复方薤白胶囊对过氧化氢(H2O2)诱导的凋亡内皮细胞的保护作用,及相关凋亡基因表达的影响,以明确其作用机制。方法:体外培养人脐静脉内皮细胞(HUVEC),以H2O2行内皮细胞凋亡造模后,再加入不同浓度的复方薤白胶囊提取液作用24h。以MTT法检测细胞增殖率,Hoechst染色观察细胞凋亡,流式细胞仪检测凋亡率,Western Blot检测抗凋亡基因Bcl-2含量变化。结果:H2O2造模后,细胞增殖明显受抑,Hoechst染色见大量凋亡细胞,流式细胞检测出明显凋亡峰,抗凋亡基因Bcl-2含量降低,与正常组相比P<0.01,具有明显的统计学意义。而各中药组提高了造模后细胞增殖率,降低了凋亡细胞及凋亡峰,提高了Bcl-2含量表达,且与浓度呈正相关,具有明显的统计学意义。结论:复方薤白胶囊能上调抗凋亡基因Bcl-2的表达,保护内皮细胞,抗内皮细胞凋亡。Objective ： To study the protective effect of Compound Macrostem Onion Capsule （CMOC） on apoptosis induced by hydrogen dioxide （H2O2 ）and apoptosis-related genes expression in human umbilical vascular endothelial cell （HUVEC） ,and reveal the mechanism of anti-apoptosis. Methods： HUVEC were cultured in vitro, and apoptosis models were established by H2O2. CMOC extract were used to culture cells induced by H2O2. Use MTr assay to measure the proliferation of the cells. The presence of apoptosis were detected by flow eytometry and Hoechst staining. Genes Bcl - 2 were detected by Western blotting. Results：Induced by H2O2 , proliferation of HUVEC and Bcl-2 expression were inhibited, and apoptosis were identified by Hoechst staining and flow cytometry. After treated by CMOC extract ,the proliferation of HUVEC was increased, apoptosis rate decreased clearly, and expression of Bcl-2 were up-regulated. All this have positive correlation with dose of CMOC and significant different from models group. Conclusion ： CMOC can protect the HUVEC against apoptosis by up regulating the expressin of Bcl-2 genes.

关 键 词：复方薤白胶囊 人脐静脉内皮细胞 过氧化氢 凋亡 BCL-2基因 

分 类 号：R285.6[医药卫生—中药学]