苦参碱对NCI-N87胃癌细胞增殖凋亡及bcl-2蛋白表达的影响  

Effects of Matrine on Proliferation/Apoptosis and bcl-2 Protein Expression of Human Gastric Cancer Cell Line NCI-N87

作　　者：王中林[1] 蒋平[2] 谭映霞[3] 于永洋[2] 潘忠良[1] 

机构地区：[1]温州市第二人民医院普外科,浙江温州325027 [2]温州医学院附属第二医院普外科,浙江温州325027 [3]温州医学院附属第一医院血液科,浙江温州325000

出　　处：《中华中医药学刊》2009年第1期141-144,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅资助项目(2000A037);温州市科技局资助项目(S990323-3)

摘　　要：目的:探讨不同浓度苦参碱对体外培养的人胃癌细胞NCI-N87的增殖、凋亡及bcl-2蛋白表达的影响。方法:NCI-N87细胞经不同浓度苦参碱处理后,采用CCK-8法检测药物对肿瘤细胞的抑制作用,显微镜观察细胞形态学改变;应用流式细胞仪分析苦参碱对细胞凋亡和bcl-2蛋白的影响。结果:苦参碱浓度>3mg/mL,细胞毒作用较大;浓度在0.5～2.0mg/mL时,对肿瘤细胞的生长有抑制作用,且呈时间剂量依赖性;浓度在0.1mg/mL,对肿瘤细胞生长几乎无抑制作用。流式细胞仪分析结果显示,细胞凋亡百分率随着药物浓度的增加和作用时间的延长而增加,而bcl-2蛋白则随之下降。结论:苦参碱对人胃癌细胞NCI-N87增殖有抑制作用,并能诱导细胞凋亡,下调bcl-2蛋白水平。Objective ：To investigate the effects of different concentrations of matrine on proliferation/apoptosis and bcl - 2 protein expression of human gastric cancer cell line NCI - N87 cultured in vitro. Methods ： The NCI - N87 cells were treated by matrine in different concentrations and variable durations. Cell growth and proliferation were observed using CCK - 8 assay. Morphological changs of cell were observed with invert microscope. Flow cytometry was used to assay the effects of matrine on apoptosis and bcl - 2 protein expression. Results： When the drug concentration was higher than 3. 0mg/mL,the major effect was cell necrosis. Matrine in the concentrations from 0.5mg/mL to 2.0mg/mL could inhibit proliferation of NCI - N87 cells as revealed by CCK - 8 assay. FCM detection showed matrine could induce apoptosis of NCI - N87 cell. The apoptosis rate of NCI - N87 cell was significantly augmented and bcl - 2 expressions decreased while the concentration and duration of matrine increased（ P 〈 0.05 ） respectively. Conclusion ： Matrine can efficiently inhibit the proliferation and induce apoptosis of NCI - N87 cell, down - regulate bcl - 2 protein expression which are the possible mechanisms for its anti - cancer effect.

关 键 词：苦参碱 胃癌细胞 细胞凋亡 BCL-2 Annexin-V/PI 

分 类 号：R285.5[医药卫生—中药学]