阳和汤含药血清对MCF-7细胞和MDA-MB-231细胞增殖抑制作用的实验研究  

Study on The Effects of Inhibiting Proliferation of Rat's Medicament Blood Serum of YH Decoction on Human Breast Cancer Cell MCF-7 ER(+) and MDA-MB-231 ER(-)

作　　者：刘亚娴[1] 田君[2] 

机构地区：[1]河北医科大学第四医院中医科,河北石家庄050011 [2]河北医科大学中西医结合学院儿科教研室,河北石家庄050091

出　　处：《中华中医药学刊》2009年第2期243-246,共4页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:鉴于清代著名医家王洪绪和马培之对阳和汤能否治疗乳岩(乳腺癌)持不同观点,通过实验探讨阳和汤用于治疗乳腺癌的可行性。方法:通过体外实验探讨阳和汤大鼠含药血清对人类乳腺癌ER(+)细胞MCF-7和人类乳腺癌ER(-)细胞MDA-MB-231的增殖抑制作用,并通过流式细胞仪检测细胞周期分布和细胞凋亡。结果:阳和汤对MCF-7细胞和MDA-MB-231细胞均有抑制作用,呈剂量和时间依赖性,并能影响细胞周期分布,诱导肿瘤细胞凋亡。但是阳和汤对ER(-)的MDA-MB-231细胞的抑制和杀伤作用远比ER(+)的MCF-7细胞要强。结论:阳和汤可以运用于乳腺癌的治疗,但是更适用于ER(-)的乳腺癌患者。Objective：Respecting the different standpoint of great Qing Dynasty physician WangHongxu and MaPeizhi about whether YangHe（YH） Decoction could cure breast cancer,we carried out the experiment.So as to investigate the feasibility of YH Decoction treat Breast cancer.Methods：To study the effects of inhibiting proliferation of rat＇s medicament blood serum of YH Decoction on human breast cancer cell MCF-7 ER（＋） and MDA-MB-231 ER（-）.Apoptosis and distribution of cell cycle were examined by flow cytometry in MCF-7 cell line and MDA-MB-231 cell line treated with different doses of medicament blood serum of YH Decoction for 48 hours.Results：YH Decoction could inhibit proliferation of MCF-7 cell and MDA-MB-231 cell in vitro by a dose dependent and time-dependent manner within a certain concentration.It also could induce an arrest of cell in cycle in G0/G1 Phase as well as apoptosis in the MCF-7 cell and the MDA-MB-231 cell.But the effect on MDA-MB-231 cell was stronger than that on MCF-7 cell.Conclusion：YH Decoction was really suitable for the breast cancer which type was ER（-）.

关 键 词：阳和汤 乳腺癌 增殖 凋亡 MCF-7 MDA-MB-231 

分 类 号：R285.5[医药卫生—中药学]