青心酮对内皮祖细胞数量和功能的影响  

The Effect of 3,4-Dihydroxyacetophenone on Endothelial Progenitor Cells

作　　者：刘小利[1] 程胜[1] 黄艳君[1] 周洁[1] 黄引平[1] 熊春秋[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000

出　　处：《中华中医药学刊》2009年第2期402-405,共4页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:观察青心酮对内皮祖细胞的影响。方法:采用密度梯度离心法从脐血中获取单个核细胞,将其接种在人纤维连接蛋白包被培养板,培养7天后,收集贴壁细胞,加入不同浓度青心酮(分别为10-4、10-5、10-6mol/L)培养一定的时间(6、12、24、48,72 h)。通过激光共聚焦显微镜鉴定FITC-UEA-I和DiI-acLDL双染色阳性细胞被认为是正在分化的EPCs。然后分别采用MTT比色法、改良的Boyden小室、黏附能力测定实验观察EPCs的增殖能力、迁移能力及黏附能力。结果:青心酮显著增加EPCs集落形成数,并且EPCs集落形成数随青心酮浓度增加及作用时间延长而增加,10-4mol/L浓度青心酮作用72h对EPCs数量的影响最为显著。青心酮也显著改善了脐血EPCs的黏附能力、迁移能力和增殖能力。结论:结果提示青心酮可增加EPCs的数量且伴随着EPCs功能的改善。Objective：To investigate the effect of 3,4-Dihydroxyacetophenone on endothelial progenitor cells.Methods：EPCs were isolated from human umbilical cord blood.Cytochemical analysis was conducted after culture for 7 days.EPCs were characterized as that DiI-acLDL/FITC-UEA-I doublepositive cells were detected by laser confocalmicroscopy.These cells were Separated into 4 groups randomly：①control group：EPCs were suspended in endothelial cell basal medium（M199）supplemented with 20% fetal calf serum;②three groups with different concentration of 3,4-dihydroxyacetophenone：EPCs were suspended in M199 supplemented with10-4,10-5,10-6mol/L 3,4-Dihydroxyacetophenone,respectively.All groups were intervented for6,12,24,48 and 72hours,To compare the number of CFU,adhesion,migration and proliferation capability of EPCs.Results：10-4mol/L 3,4-Dihydroxyacetophenone markedly increase number of CFU,migration,adhesion and proliferation capability of EPCs with time accrescence,and it display time dependence.These parameters reach the peak at 72 hours.Conclusion：3,4-dihydroxyacetophenone may promote the repair of the damaged blood vessel endothelium and vasculogenesis through effecting the quantity,migration adhesion and proliferation capability of EPCs.

关 键 词：青心酮 内皮祖细胞 妊娠期高血压疾病 

分 类 号：R282.7[医药卫生—中药学]